The relationship between the primary cell wall phenolic acids, dehydrodimers of ferulic acid, and maize grain resistance to Fusarium graminearum, the causal agent of gibberella ear rot, was investigated. Concentrations of dehydrodimers of ferulic acid were determined in the pericarp and aleurone tissues of five inbreds and two hybrids of varying susceptibility and in a segregating population from a cross between a resistant and susceptible inbred. Significant negative correlations were found between disease severity and diferulic acid content. Even stronger correlations were observed between diferulic acid and the fungal steroid ergosterol, which is an indicator of fungal biomass in infected plant tissue. These results were consistent over two consecutive field seasons, which differed significantly for temperature and rainfall during pollination, the most susceptible stage of ear development. No correlation was found between the levels of these phenolics and deoxynivalenol levels. This is the first report of in vivo evidence that the dehydrodimers of ferulic acid content in pericarp and aleurone tissues may play a role in genotypic resistance of maize to gibberella ear rot.

2. Two fully-factorial mesocosm experiments (one for each crayfish pair) were used to investigate crayfish diet, and their impact on benthic invertebrate community structure, benthic algal standing stock and leaf litter decomposition rates, in allopatric and sympatric populations, compared to a crayfish free control. We used stable isotope analysis to examine crayfish diet in the mesocosms and in allopatric populations of each species in the Thames catchment.3. Isotopic niche width did not vary significantly between allopatric and sympatric populations of crayfish pairs in the mesocosms and isotopic niche partitioning in all the wild populations suggests the invaders can coexist.4. All four species altered benthic invertebrate community structure but with differing functional effects, often mediated via trophic cascades. Red swamp crayfish predation upon snails evidently promoted benthic algal standing stock via reduction in grazing pressure. However, a trophic cascade whereby the crayfish consumed native invertebrate shredders, causing a reduction in net leaf litter decomposition, was decoupled by red swamp and signal crayfish since they consumed leaf litter directly and thus moderated the cascade to a trickle when in sympatry with Turkish or virile crayfish, respectively. 6. Our results suggest that the combined effect of multiple invasions on the ecosystem can reflect either an additive effect of their independent impacts, or an amplified effect, which is greater than the sum of their independent impacts. A lack of general pattern in their effects makes any potential management strategy more complex.

Gibberella ear rot, caused by the fungus Fusarium graminearum Schwabe, is a serious disease of corn (Zea mays) grown in northern climates. Infected corn is lower yielding and contains toxins that are dangerous to livestock and humans. Resistance to ear rot in corn is quantitative, specific to the mode of fungal entry (silk channels or kernel wounds), and highly influenced by the environment. Evaluations of ear rot resistance are complex and subjective; and they need to be repeated over several years. All of these factors have hampered attempts to develop F. graminearum resistant corn varieties. The aim of this study was to identify molecular markers linked to the genes for resistance to Gibberella ear rot. A recombinant inbred (RI) population, produced from a cross between a Gibberella ear rot resistant line (CO387) and a susceptible line (CG62), was field-inoculated and scored for Gibberella ear rot symptoms in the F4, F6, and F7 generations. The distributions of disease scores were continuous, indicating that resistance is probably conditioned by multiple loci. A molecular linkage map, based on segregation in the F5 RI population, contained 162 markers distributed over 10 linkage groups and had a total length of 2237 cM with an average distance between markers of 13.8 cM. Composite interval mapping identified 11 quantitative trait loci (QTLs) for Gibberella ear rot resistance following silk inoculation and 18 QTLs following kernel inoculation in 4 environments that accounted for 6.7%-35% of the total phenotypic variation. Only 2 QTLs (on linkage group 7) were detected in more than 1 test for silk resistance, and only 1 QTL (on linkage group 5) was detected in more than 1 test for kernel resistance, confirming the strong influence of the environment on these traits. The majority of the favorable alleles were derived from the resistant parent (CO387). The germplasm and markers for QTLs with significant phenotypic effects may be useful for marker-assisted selection to incorporate Gibberella ear rot resistance into commercial corn cultivars.

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Coastal steepening potentially presents an array of management issues in the form of financial implications of sea defence degradation, increased risk posed to the hinterland as wave attenuation is reduced, 'coastal squeeze' and statutory requirements in the light of the Habitats Directive. The extent to which coastal steepening has occurred throughout England and Wales has been investigated through use of a GIS and dataset based on historical Ordnance Survey map information. Data were collected along 1084 selected profile lines, positioned so as to be geomorphologically representative of the coast. Features recorded from each map year included the positions of mean high water (MHW) and mean low water (MLW), the relative movements of which infer changing intertidal gradients. The results presented in this paper are on a subject and scale not previously published. It is revealed that 61% of the coastline studied has experienced a tendency towards steepening. Of the remainder, 33% has flattened, and 6% has experienced no rotational movement. This tendency towards steepening has been the dominant movement on each of the west, south, and east coasts.