A new simple, selective, rapid, precise and accurate reverse phase HPLC method has been developed for simultaneous estimation of granisetron and dexamethasone. The method was developed using CPS Hypersil CN column (250×4.6 mm I.D.) with a mobile phase consisting of acetonitrile:buffer (100 mM Triethylamine adjusted to pH 3.0 with o-phosphoric acid) in ratio of 25:75 at a flow rate of 2 ml/min. Detection was carried out at 242 nm. The developed method was evaluated for various system suitability parameters and validated for linearity, accuracy, precision, LOD, LOQ as per ICH guidelines. It was also evaluated for bench top stability and freeze/thaw stability. The proposed method can be used for the estimation of these drugs in their combined dosage forms.
The seroprevalence study of peste des petits ruminants (PPR) in sheep and goats was carried out in 5 districts of Saurashtra region of Gujarat state. A total of 828 (prevaccinated) and 423 (postvaccinated) serum samples from sheep and goats were screened for PPR specific antibodies using PPR specific c-ELISA. Overall seroprevalence of PPR was found to be 53.86 (446/828) and 72.34 (306/423) % in pre- and post-vaccinated small ruminants, respectively. Overall pre-/post-vaccinated seroprevalance rates were highest in Bhavnagar district (83.89/96.30 %), followed by Surendranagar (73.61/77.78 %), Amreli (56.25/83.33 %), Jamnagar (32.22/43.33 %) and Rajkot (27.78/64.44 %) districts. Pre and post vaccination seropositivity was noted higher in goats (59.90/78.75 %) than in sheep (35.75/52.43 %). Males in both the species (goats and sheep) showed higher seroprevalence (60.11/89.01 %) as compared to their female counterparts (52.09/67.77 %). Higher prevaccinated seroprevalence was recorded among age group of 1 to 2 years (63.41 %), followed by below 1 year age group (53.99%) and above 2 years of age group (44.20%).
A stable, simple, rapid, precise, accurate RP-HPLC method for simultaneous analysis of diclofenac sodium and rabeprazole sodium was developed and validated as per ICH guidelines without need of any internal standard. Separation was carried out using C8 column with triethyl amine buffer (pH 5): acetonitrile (50:50 v/v) as mobile phase with flow rate 2 mL min -1 . The detection was carried out at 284 nm. The parameters studied were retention time, linearity and range, accuracy, precision, detection limit, quantitation limit and bench top stability. The proposed method can be used for simultaneous estimation of diclofenac sodium and rabeprazole sodium in bulk drugs and pharmaceutical dosage forms.
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