BackgroundEndometriosis, pro-inflammatory and invasive benign disease estrogen dependent, abnormally express in endometria the enzyme P450Arom, positively regulated by steroid factor-1 (SF-1). Our objective was to study the nuclear protein contents of upstream stimulating factor 2 (USF2a and USF2b), a positive regulator of SF-1, throughout the menstrual cycle in eutopic endometria from women with and without (control) endometriosis and the involvement of nuclear estrogen receptors (ER) and G-coupled protein estrogen receptor (GPER)-1.ResultsUpstream stimulating factor 2 protein contents were higher in mid (USF2b) and late (USF2a and USF2b) secretory phase in eutopic endometria from endometriosis than control (p < 0.05). In isolated control epithelial cells incubated with E2 and PGE2, to resemble the endometriosis condition, the data showed: (a) significant increase of USF2a and USF2b nuclear protein contents when treated with E2, PPT (specific agonist for ERα) or G1 (specific agonist for GPER1); (b) no increase in USF2 binding to SF-1 E-Box/DNA consensus sequence in E2-treated cells; (c) USF2 variants protein contents were not modified by PGE2; (d) SF-1 nuclear protein content was significantly higher than basal when treated with PGE2, E2 or G1, stimulation unaffected by ICI (nuclear ER antagonist); and (e) increased (p < 0.05) cytosolic protein contents of P450Arom when treated with PGE2, E2, PPT or G1 compared to basal, effect that was additive with E2 + PGE2 together. Nevertheless, in endometriosis cells, the high USF2, SF-1 and P450Arom protein contents in basal condition were unmodified.ConclusionThese data strongly suggest that USF2 variants and P450Arom are regulated by E2 through ERα and GPER1, whereas SF-1 through GPER1, visualized by the response of the cells obtained from control endometria, being unaffected the endogenously stimulated cells from endometriosis origin. The lack of E2 stimulation on USF2/SF-1 E-Box/DNA-sequence binding and the absence of PGE2 effect on USF2 variants opposite to the strong induction that they exert on SF1 and P450 proteins suggest different mechanisms and indirect regulations. The sustained USF2 variants protein expression during the secretory phase in eutopic endometria from women with endometriosis may participate in the pathophysiology of this disease strongly associated with infertility and its characteristic endometrial invasion to ectopic sites in the pelvic cavity.

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A new method for the treatment of chronic pancreatitis by introducing into arterial bed of pancreas or a regional arterial bed pancreas suspension of hydrocortisone. Objective: To provide local intensification anti-inflammatory effects, reducing the risk of systemic side effects. Material and methods: Endovascular treatment of 30 patients (19 males (63,3%) and 11 women (36.7%), the average age of 50.7 years. Organ (gastroduodenal artery) or regional (celiac trunk) infusion of a suspension of hydrocortisone. Indications e ineffectiveness of conservative therapy, recurrent of pain.Clinical evaluation of the dynamics of abdominal pain during the infusion, within 1 month and 6 months. A score of pain, where 0-his absence, the maximum intensity of 5.Studied indices of tissue blood flow in the stomach by a method rheoarteriogastrography at baseline, at the middle and at the end of long arterial infusion suspension of hydrocortisone in pool celiac trunk, n = 15.We studied the performance of the specific density of the pancreatic tissue during the treatment. Results: Severe pain at the time of admission to the surgical hospital. After endovascular treatment of pain intensity reduced to 0,34 AE 0,19 points.Flow condition in the stomach without ischemic changes during and after treatment.According to the CT study noted a decrease in the size of the pancreas, the restoration of the specific density of the parenchyma to normal values. Conclusions: Endovascular treatment method allows HP to stop inflammation, affect tissue fibrosis and the loss of its basic functions.

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