No abstract

Jerabek J., L. Dedek: Titration of the Shope Fibroma Virus in vitro. Acta vet. Bmo, 50, 1981: 89-96. For titration of the Shope fibroma virus propagated in cell line RK 13 besides rabbits also the cell culture line RK 13 may be: used. Titration in vitro by both the test tube method and micromethod in platea can replace the laborious titration iri rabbits employed up to now. The mjCJ:omethod proved to be more advantageous as it is simple and time-saving. Titration in vitro may be used to test any phase, of vaccine production, a fact important not. only for vaccine standardization"but also (or production economy.

No abstract

Cell lines IBRS-2, PK-C or primary porcine kidney cells are the most suitable ones for AujeszkY's disease virus propagation for preparation of an inactivated vaccine. Glutaraldehyde-inactivated virus of the Aujeszky's disease proved to be safe in mice and rabbits. Effectiveness of the vaccine was tested in rabbits. Their vaccination followed by revaccination generated immunity for 6 months. Lyophilized inactivated vaccine can be stored for long periods without loss of effectiveness. The vaccine is reconstituted immediately before use by the supplied diluting fluid containing a lipoid adjuvant.

In blood sera of rabbits, guinea-pigs, laboratory rats and white mice, specific antibodies were detected after vaccination and revaccination with an inactivated vaccine against porcine parvovirosis using the haemagglutination inhibition test. The strongest immune response was found in laboratory rats showing haemagglutionation inhibiting antibody titres in the range of 256-2048; guinea-pigs also showed aubstantial titres in the range of 16--1024. Lriwer immune respcinses were detected with white mice (titres 16-32) and rabbits (titres 8-128). These results indicate that the most suitable laboratory animals for efficacy testing of inactivated vaccines against porcine parvovirosis are· laboratory rats and guinea-pigs.