Titration of the Shope Fibroma Virus in vitro

Jeřábek, J.; Dedek, L.

doi:10.2754/avb198150010089

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2017

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“…The cytopathic effect (CPE) associated with influenza virus infection may be an useful indicator of peaked virus production [ 21 ]. Measurement of tetrazolium formazan products by MTS or MTT assay is frequently used for evaluation of the level of cell viability [ 22 , 23 ].…”

Section: Resultsmentioning

confidence: 99%

Distinct susceptibility and applicability of MDCK derivatives for influenza virus research

et al. 2017

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Madin-Darby Canine Kidney (MDCK) cells are widely utilized as a substrate for influenza virus isolation and propagation due to the high yields of virus. Here we compared the conventional MDCK cell line, MDCK-SIAT1 and MDCK-London for viral production, cell survival, and suitability in testing antivirals using six influenza strains including two H1N1 (pandemic and epidemic strains), three H3N2 and one influenza B strain. Overall our results suggest that MDCK-London cell line is superior for virus culturing and quantification, and hence an ideal platform to evaluate antiviral drug efficacy against multiple strains of influenza. Our data also suggests that while virus titers determined by the hemagglutination assay (HA) and neuraminidase activity (NA) are widely used to indicate viral load, there is a poor correlation between these measurements and the infectious titer obtained by plaque assay.

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Section: Resultsmentioning

confidence: 99%