Seven monoclonal antibodies (MAbs) were derived from mice immunized with the rabies virus glycoprotein of the Pitman-Moore (PM) strain. These antibodies recognized at least five partially overlapping sites located in one immunodominant region. A panel of MAbs was then used to characterize antigenic relationship between PM strain and SAD-Vnukovo strain of these rabies viruses. In immunoblot, all tested antibodies bound to the glycoprotein of both rabies strains, indicating shared antigenic determinants located on the corresponding immunodominant regions. The pattern of reactivity in immunoblot suggested the specificity of antibodies against linear epitopes. However, the supposed close antigenic relation between PM and SAD-Vnukovo strains (evidenced by immunoblot) was not fully confirmed by immunoenzymatic assay. Data provided by ELISA demonstrated two distinct patterns of MAbs reactivity with both antigens. Four antibodies showed specificity for PM strain glycoprotein only, while three MAbs bound with both PM and SAD-Vnukovo strain antigens. We supposed the strain-specific conformation of the native glycoprotein to be responsible for selective access of single MAbs to the respective common linear epitopes.

Summary A rhabdovirus isolated from carp tissues during an epizootic of the acute form of infectious dropsy (spring viraemia) of carp multiplied and produced cytopathic effects in cell lines FBM, BB and EPC. The virus showed typical rhabdovirus morphology. Zusammenfassung Isolierung und Elektronenmikroskopie eines Rhabdovirus von der akuten Form der infektiösen Bauchwassersucht der Karpfen (Spring Viraemia of Carp) Während einer Epizootie der akuten Form der Bauchwassersucht (Spring Viraemia) wurde von Karpfengeweben ein Rhabdovirus isoliert, das sich mit einem cytopatnischen Effekt in den Zellinien FHM, BB und EPC vermehrte. Das Virus besaß die typische Morphologie der Rhabdoviren.

Jerabek J., L. Dedek: Titration of the Shope Fibroma Virus in vitro. Acta vet. Bmo, 50, 1981: 89-96. For titration of the Shope fibroma virus propagated in cell line RK 13 besides rabbits also the cell culture line RK 13 may be: used. Titration in vitro by both the test tube method and micromethod in platea can replace the laborious titration iri rabbits employed up to now. The mjCJ:omethod proved to be more advantageous as it is simple and time-saving. Titration in vitro may be used to test any phase, of vaccine production, a fact important not. only for vaccine standardization"but also (or production economy.

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Cell lines IBRS-2, PK-C or primary porcine kidney cells are the most suitable ones for AujeszkY's disease virus propagation for preparation of an inactivated vaccine. Glutaraldehyde-inactivated virus of the Aujeszky's disease proved to be safe in mice and rabbits. Effectiveness of the vaccine was tested in rabbits. Their vaccination followed by revaccination generated immunity for 6 months. Lyophilized inactivated vaccine can be stored for long periods without loss of effectiveness. The vaccine is reconstituted immediately before use by the supplied diluting fluid containing a lipoid adjuvant.