The responses of four mushroom cultivars ('PSU-310,' 'PSU-324,' 'PSU-348,' and 'PSU-344') exposed to free-living nematode infestations were compared in two experiments by quantitatively determining the amount of mycelium in compost samples using a laccase assay technique. The amount of mycelial production for cultivars 'PSU-310,' 'PSU-324,' and 'PSU-348' in the control treatments of both studies was similar. While cultivar 'PSU-344' produced less mycelium as shown by laccase production, mycelial production was greatly reduced for all four cultivars exposed to nematode infestations in a high-moisture compost (78%) over the duration of the experiment. The initial compost moisture is implicated as a critical factor influencing the potential damage caused by nematode infestations.
A technique is described for the quantitative separation of very low concentrations of carbohydrates by gas–liquid chromatography using polar and nonpolar silicone liquid phases and TMS (trimethylsilyl) derivatives.
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