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Sun-dried calyxes of Hibiscus sabdariffa were finely grinded and fractionated by controlled differential sieving processing (CDSp) into four granulometric classes. The obtained powder fractions were examined for their micronutrient contents and in vivo antioxidant properties in high-fat diet induced oxidation model rats. Unsieved powder and lyophilized ethanolic extract were used for comparison. Micronutrient contents and antioxidant properties were significantly influenced (p \ 0.05) by particle sizes. The mineral, vitamin, carotenoid and anthocyanin contents were shown to be higher in smaller powder particles. Comparatively, the ethanolic extract powder had the higher carotenoid, vitamin and anthocyanin contents while the superfine powders \ 180 lm and 180-212 lm had the higher mineral contents and antioxidant properties which was characterized by high superoxide dismutase, catalase activities and low malondialdehyde production. Plant grinding followed by CDSp may be an option to improve micronutrient content and antioxidant activity of plant powders.

Cochlospermum tinctorium A. Rich. (Bixaceae) is a widespread herbaceous plant in the African sahelian and soudanian zones. Its root is used as food and medicinal plant. Studies of secondary metabolites, functional activities and trichromatic parameters were realized according to standard methods of laboratory. At a threshold of α = 0.05, significant correlations (p < 0.05) were found between secondary metabolites contents and functional activities such as radical scavenging activity of antioxidant component or hydroxyl radical scavenging activity. The value of DPPHH was 95.56 ± 0.94% for CT inner Matrix with variation of 0.12% for CT Soaked inner Matrix and 1.63% for CT Peeler. But HSRA was 37.14 ± 5.71 for CT inner Matrix with variation of-38.46% for CT Soaked inner Matrix and-71.79% for CT Peeler. Cochlospermum tinctorium root has exhibited efficiency to heal many diseases thanks to multiple bioactive compounds. In addition, the colour of powders depended upon chemical compounds. Finally, Cochlospermum tinctorium root was found to neutralize organic radicals and reactive oxygen species.

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