The results of previous studies have been used to calculate the economically optimal reaction conditions in the production of high fructose syrup by isomerization over an Immobilized catalyst In a packed bed reactor. It is found that the optimum pH of the feed Is 7.65. The sensitivity of the total operation costs for small variations in the pH (0.1 pH unit) Is small. The temperature should preferably He between 50 and 55 °C or as close as possible to this range while avoiding microbiological growth In the system. The catalyst particles should be so small that their effectiveness factor Is unity. If unity cannot be reached for practical reasons, the particles should be as small as the pressure drop over the reactor allows.

Ind. Eng. Chem. Process Des. Dev. 1983, 22, 349-356 349 Guln, J. A.; Tarrer, A. R.; Lee, J. M.; Lo, L.; Curtls, C. W. Ind. Eng. Chem. Process Des. Dev. 1979, 18, 371. Guln, J. A.; Tarrer, A. R.; Lee, J. M.; Van Brackle, H. F.; Curtis, C. W. Ind. Eng. Chem. Process Des. Dev. 1970, 18, 631. Guin, J. A.; Lee, J. M.; Fan, C. W.; Curtis, C. W.; Lloyd, J. L.; Tarrer, A. R. Ind. Eng. Chem. Process Des. Dev. 1980, 19, 440. Hung. W. J.; Curtis, C. W.; Guin, J. A.; Clinton, J. H.; Barwood, H. L.; Tarrer, A. R. Paper 6d presented at Sprlng National AlChE Meetlng, Houston, TX, 1981a. Hunag, W. J.; Smith, N. L.; Guin, J. A.; Curtis, C. W.; Tarrer, A. R. Presented at 74th Annual AlChE Meeting, New Orleans, LA 1981b. Lambert. J. M.; Slmkovlch, 0.; Walker, P. L. Fuel 1980, 59, 687. Lowry, H. H., Ed. "Chemlstry of Coal Utilizatlon", Supplementary Volume; Wlley: New York; 1963; p 265-268. MazzOCco, N. J.; Klunder, E. 8.; Krastman, D. Pittsburgh Eng. Tech. Center, Phburgh, PA, Report No. DOE/PETC/Tl?-81/1, March 1981. Montano, P. A.; Granoff, B. Fuel 1980. 59. 214. Moroni, E. C. Paper 8a presented at Sprlng AIChE Meeting, Houston, TX, 1981. Neavei, R. C. Fuel 1976, 55, 237. Neavel, R. C. Phil. Trans. R . Soc. Lond. 1981. A300. 141. Orchin, M.; Storch, H. H. Ind. €ng. Chem. 1948, 40, 1385. Raaen, V. F.; Roark, W. H. Fuel 1978, 57. 650. Sapre, A. V.; Gates, B. C. Ind. €ng. Chem. Process Des. Dev. 1981, 20, Thomas, M. G.; Padrlck, T. D.; Stohi, F. V. Sandla Lab. Report SAND-80-Varghese, P.; Derbyshke, F. J.; Whltehurst, D. C. Paper 6b presented atThe rate of deactivation of an immobilized whole cell Arthrobacter glucose isomerase catalyst has been determined quantitatively as a function of a number of parameters. The deactivation is most senskive to pH and temperature, while other possible parameters, such as oxygen concentration, byproduct Concentration, and impurities present in a synthetic feed stream, appeared to have only a minor Influence on the rate of deactivation under normal process conditions. The deactivation is probably caused by denaturation of the enzyme and not by mechanisms such as proteolysis, change of the membrane structure of an Arthrobacter cell, or pore plugging.