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Daidzein, not previously reported in forages, was found in alfalfa, and ladino, red, and subterranean clovers. Formononetin and genistein, isolated earlier from red and subterranean clovers, have now also been found in alfalfa and ladino clover. Biochanin A, previously reported in red and subterranean clovers, was found in alfalfa but not in ladino clover. A combination of three sequential purifications on paper chromatograms followed by one on silicic acid chromatostrips was used to isolate the isoflavones. The identity of the compounds was confirmed by absorption spectra as well as by comparison of paper and silicic acid chromatograms of the isolated compounds with those of authentic samples of the isoflavones.NTEREST in naturally occurring forage

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