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cis-Jasmone, or (Z)-jasmone, is well known as a component of plant volatiles, and its release can be induced by damage, for example during insect herbivory. Using the olfactory system of the lettuce aphid to investigate volatiles from plants avoided by this insect, (Z)-jasmone was found to be electrophysiologically active and also to be repellent in laboratory choice tests. In field studies, repellency from traps was demonstrated for the damson-hop aphid, and with cereal aphids numbers were reduced in plots of winter wheat treated with (Z)-jasmone. In contrast, attractant activity was found in laboratory and wind tunnel tests for insects acting antagonistically to aphids, namely the seven-spot ladybird and an aphid parasitoid. When applied in the vapor phase to intact bean plants, (Z)-jasmone induced the production of volatile compounds, including the monoterpene (E)--ocimene, which affect plant defense, for example by stimulating the activity of parasitic insects. These plants were more attractive to the aphid parasitoid in the wind tunnel when tested 48 h after exposure to (Z)-jasmone had ceased. This possible signaling role of (Z)-jasmone is qualitatively different from that of the biosynthetically related methyl jasmonate and gives a long-lasting effect after removal of the stimulus. Differential display was used to compare mRNA populations in bean leaves exposed to the vapor of (Z)-jasmone and methyl jasmonate. One differentially displayed fragment was cloned and shown by Northern blotting to be up-regulated in leaf tissue by (Z)-jasmone. This sequence was identified by homology as being derived from a gene encoding an ␣-tubulin isoform.
Smart cities have been recently pointed out by M2M experts as an emerging market with enormous potential, which is expected to drive the digital economy forward in the coming years. However, most of the current city and urban developments are based on vertical ICT solutions leading to an unsustainable sea of systems and market islands. In this work we discuss how the recent vision of the Future Internet (FI), and its particular components, Internet of Things (IoT) and Internet of Services (IoS), can become building blocks to progress towards a unified urban-scale ICT platform transforming a Smart City into an open innovation platform. Moreover, we present some results of generic implementations based on the ITU-T's Ubiquitous Sensor Network (USN) model. The referenced platform model fulfills basic principles of open, federated and trusted platforms (FOTs) at two different levels: the infrastructure level (IoT to support the complexity of heterogeneous sensors deployed in urban spaces), and at the service level (IoS as a suit of open and standardized enablers to facilitate the composition of interoperable smart city services). We also discuss the need of infrastructures at the European level for a realistic large-scale experimentally-driven research, and present main principles of the unique-in-the-world experimental test facility under development within the SmartSantander EU project.
The experiments described have given the following conclusions: (I) A chemical signal (pheromone) emitted by the oviparous female has an attractive effect upon the males. This signal is not emitted by the virginogeniae. (2) The signal is emitted by organs situated on the hind tibiae of the oviparous female and perceived by organs on the male antennae. (3) The substance is only emitted by the females during a certain period in their lifetime. Apparently, this is principally the condition which has been called active copulatory period (ACP). No emittance was found neither during the passive precopulatory period (PPP) nor during the passive postcopulatory period (PCP). (4) Males are capable of detecting and reacting to the signal already in the last two larval instars. (5) The males do not emit any substance affecting other males. Statistical analysis (Student's t-test) of the material has been excluded as it gave significance only in experiments where differences between the stimuli are apparent.
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