Aims: This study was assessed to demonstrate the antimicrobial activity in vitro of an identified fluorescent Pseudomonas strain characterized for its capacity to produce phenazine compounds. Methodology: First Pseudomonas chlororaphis subsp aureofaciens was inoculated on Nutrient Broth supplemented with Yeast Extract (NBY) and with glucose at a final concentration of 2%, after incubation the filtered culture was acidified with HCl to pH 2. The solution was extracted twice with the same volume of ethyl-acetate. The organic supernatants were combined, dried over anhydrous Na 2 SO 4 , and evaporated to dryness. The crude extract was resuspended in methanol and tested
This work was carried out in collaboration between all authors. Author SMA designed the study, wrote the protocol and interpreted the data. Authors SMA and NH anchored the field study, gathered the initial data and performed preliminary data analysis. Authors SMA, NH and MMZ managed the literature search, produced the initial draft and wrote the manuscript, while authors MMZ, JN and AG read and approved the publication. All authors read and approved the final manuscript.
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