Human toxocariasis may be acquired by eating raw chicken liver. However, there are no reports on the prevalence of natural infection of chickens with Toxocara. The aim of this study was to evaluate the presence of anti-Toxocara antibodies as indicators of natural infection with Toxocara, in free-range chickens from Espírito Santo State, Brazil. An ELISA test with secretory and excretory Toxocara canis antigens was used. Negative controls were 20 industrial chickens reared in a high hygiene standard environment. Positive control serum was from a chicken infected with embryonated eggs of T. canis. Sera were adsorbed with Ascaridia galli extract to reduce cross-reactivity. Cut-off was the mean plus four times the standard deviation of optical density (OD) in negative group. One hundred and fifty-seven sera from free-range chicken were investigated. Results showed 58.5% of the chickens were positive with ELISA test; 12.7% had OD over the positive control and may be considered as true infected chickens. The results between the cut-off and the positive control may include infections with low titers of antibodies or may represent serum scar of past infection or may be the result of cross-reaction with other nematodes rather than A. galli which is used for the adsorption of sera. In conclusion, high prevalence of Toxocara sp. antibodies demonstrates natural infection of free-range chickens from Espírito Santo State which may represent a risk of infection with this nematode in people who have the habit of eating raw or undercooked chicken meat or viscera. The results also suggest that chickens may be useful as sentinels to detect soil contaminated with Toxocara eggs.

The objective of this study was to evaluate the infectivity of Toxocara canis eggs after interacting with isolated nematophagous fungi of the species Duddingtonia flagrans (AC001) and Pochonia chlamydosporia (VC4), and test the predatory activity of the isolated AC001 on T. canis second stage larvae after 7 days of interaction. In assay A, 5000 embryonated T. canis eggs previously in contact with the AC001 and VC4 isolated for 10 days were inoculated into domestic chickens (Gallus gallus domesticus), and then these animals were necropsied to collect material (digested liver, intestine, muscles and lungs) at 3-, 7-, 14-, and 21-day intervals after inoculation. In assay A, the results demonstrated that the prior interaction of the eggs with isolated AC001 and VC4 decreases the amount of larvae found in the collected organs. Difference (p < 0.01) was observed in the medium larvae counts recovered from liver, lung, intestine, and muscle of animals in the treated groups when compared to the animals in the control group. At the end of assay A, a percentage reduction of 87.1 % (AC001) and 84.5 % (VC4) respectively was recorded. In the result of assay B, the isolated AC001 showed differences (p < 0.01) compared to the control group, with a reduction of 53.4 % in the recovery of L2. Through these results, it is justified to mention that prior interaction of embryonated T. canis eggs with the tested fungal isolates were efficient in reducing the development and migration of this parasite, in addition to the first report of proven predatory activity on L2.

The present work used Plackett-Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett-Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P < 0.05) on the destruction of S. westeri larvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P < 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P < 0.05) on the destruction of P. fastosum eggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.

Platynosomum fastosum é um parasito de felinos, entre os quais os gatos domésticos, sobretudo os originados de locais onde o clima é tropical e subtropical. Este é o parasito hepático mais comum de gatos domésticos e se localiza geralmente nas vias biliares e vesícula biliar, mas também pode ser encontrado no intestino delgado e pâncreas. A infecção se dá por meio da ingestão de lagartixa contendo as metacercárias que, nas vias biliares de gatos domésticos, causam uma colangite. O objetivo do presente trabalho foi relatar a ocorrência de P. fastosum, verificada por meio de necropsia de um gato doméstico, assintomático, com histórico de morte súbita, recebido no setor de Patologia Animal do Hospital Veterinário Ricardo Alexandre Hippler, Espírito Santo, Brasil. No exame macroscópico, foram observados exemplares adultos de P. fastosum no interior dos ductos biliares que se encontravam visivelmente dilatados. No exame histopatológico, foram confirmadas dilatação, presença de parasitos e proliferações papilares para a luz dos ductos, seguindo-se a classificação como colangiohepatite associada à infecção por P. fastosum. Este é o primeiro relato da ocorrência de P. fastosum em um gato doméstico no Espírito Santo.

The objective of this study was to evaluate, in vitro, the colonization and destruction of ants of the genus sp. by the ovicidal fungus (VC4 isolate), in the southeast region of Brazil. The insects used in the experiment were worker ants of the genus sp., collected periodically in the environment and immediately transported to the laboratory in test tubes. Then, VC4 growth was promoted in 2% chitin agar medium (2% WQ) to obtain a fungal solution containing conidia and/or chlamydospores. Two experimental groups were formed. Treated group consisted of Petri dishes containing 2% agar-water culture medium (2% WA) with nine live insects and 20 µL of fungal solution at the concentration of 15,000 conidia/chlamydospores. Control group consisted of Petri dishes containing 2% WA culture medium and nine live insects. The dishes in the treated and control groups were incubated in BOD at 25 ± 1 °C and 80 ± 10% relative humidity for 4 days. After 4 days, it was observed that the VC4 had grown, colonized, and caused the destruction of the ants. The fungus was efficient at colonizing and destroying the urban ants collected on an experimental basis. Thus, it could open up new ways to reduce the use of chemical compounds in the future, decreasing health and environmental problems.