Objective: The aim of the present work was to extract the chitin and chitosan from two species of Pleurotus, its characterization and its antimicrobial activity against bacteria and fungi. Methods:The fungi were grown in MGYP medium for 15 d. The chitin and chitosan were extracted using the alkaline method and various biochemical methods and compared with commercial chitin and chitosan. The FTIR and SEM were also performed. Antimicrobial activity was performed using well diffusion method. Results:The maximum yield of mycelia and the chitin and chitosan were obtained on 12 th Conclusion:In the present study the chitin and chitosan were extracted from the Pleurotus spp. (P. florida and P. eous). The yield was maximum in P. florida after 12 day of incubation. P. folrida gave maximum yield (201.3 mg/l of chitin and 65.61 mg/l of chitosan). Fourier Transform Infrared spectroscopy comparing the results obtained with results for commercial chitin and chitosan. Our results showed that chitin and chitosan obtained from Pleurotus spp. had a significant similitude with commercial. SEM image also carried out to study the surface morphology. The fungal chitin and chitosan had more antimicrobial activity against the gram+ve, gram-ve and the various fungus.th Keywords: Chitin, Chitosan, FTIR, SEM, Pleurotus spp., Antibacterial activity day of fermentation. The FTIR and SEM also confirmed the chitin and chitosan. The extracted chitin and chitosan were subjected to antimicrobial activity, and it was found the extracted chitin and chitosan were active against the bacteria and fungi. Our findings suggest that the Pleurotus spp. are the potential candidate to produce eco-friendly chitin and chitosan in the development of drugs, artificial bone and raw material for the food industries in the near future.

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Jasmine plant species are widely grown in Asia and used for religious offering, is known to have wide range of bioactive compounds and its properties. Most of the jasmine species have been evaluated its bioactive properties and found positive results. This work consists of evaluating bioactive properties of jasmine species, Jasminum polyanthum and finding its potential medicinal uses. The plant leaves and flowers were powdered and added water to prepare extract. Both leaves and flower extract was evaluated for phytochemical compounds, anti oxidant, anti diabetic, anti inflammatory, anti microbial, anti cancer and DNA nicking assay. The leaf and flower extract contained most of the phytochemical compounds which leads to presence of various bioactive properties. Leaf possesses good DPPH activity, while flower possess high phenol content and FRAP activity.  Leaf possesses good anti diabetic activity, while flower possess good anti inflammatory activity. Flower extract consists of higher antibacterial activity than leaf, while in antifungal it is vice versa.   Keywords:  Anti-diabetic, Anti-inflammatory, Antioxidant activity, MTT Assay, DNA Nicking study

Nanotechnology is an emerging technology for the treatment of waste water. Nanoparticles have its own advantages as the higher surface area to volume ratio compared to the bulk material. In this study, zinc oxide-based nanoparticles were synthesized. Synthesized nanoparticles are characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy (EDX). The antibacterial study was carried out using the synthesized nanoparticle. The photocatalytic degradation for methyl blue, methyl red, and Orange G is also done in this study using the synthesized nanoparticles. The shape and size of the nanoparticles obtained are rounding spherical with 80 to 110 nm. The optimum result obtained from the dye degradation study is 94% for methyl blue.

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