δ-Catenin induces dendritic morphogenesis in several cells and it was reported that deletion of C-terminal 207 amino acid of δ-catenin completely abolished the dendritic morphogenesis. However, exact domain responsible for inducing dendritic morphogenesis in C-terminus of δ-catenin was not mapped. Here, we report that expression of ΔC47 (lacking 47 amino acid of C-terminus: 1-1200), ΔC77 (lacking 77 amino acid of C-terminus: 1-1170) deletion mutants of δ-catenin induced the dendritic morphogenesis of HEK293T and NIH3T3 cells as full-length δ-catenin did. In agreement with previous report, ΔC207 deletion mutant did not show the dendritic morphogenesis of the cells. Interestingly, introducing 107 amino acid deletion of C-terminus (ΔC107 mutant: 1-1140) and 177 amino acid deletion of C-terminus (ΔC177 mutant: 1-1070) showed limited primary and secondary dendritic process and notable spine-like process formation. These results suggest that 1140-1170 amino acid of C-terminal δ-catenin is required for primary and secondary dendrite-like process formation.
In this study, 15 evaluation questions about light were developed and presented to 30 middle school students, and then the theory of knowledge space was used to analyze the status of the middle school students' knowledge about light. Not only was the state of the students' knowledge about light intended to be measured by schematizing the knowledge hierarchy obtained; the data obtained were also intended to be used as basic materials to improve the teaching methods used. To achieve the purpose of this study, the evaluation results, the individual knowledge state, and the hierarchy of questions were analyzed. As a result, different knowledge structures were found in the individuals and groups, and it was determined that individuals and groups should be diagnosed differently. In addition, the implication that there is a connection between each question and the individual knowledge state was drawn.
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