Glutathione S-transferases (GSTs) constitute a large family of enzymes with a wide range of cellular functions. Recently, plant GSTs have gained a great deal of attention due to their involvement in the detoxification of electrophilic xenobiotics and peroxides under adverse environmental conditions, such as salt, cold, UV-B and drought stress. A previous study reported that a GST gene (CsGSTU8) in tea plant was distinctly induced in response to drought, suggesting this gene plays a critical role in the drought stress response. In this study, by using quantitative real-time PCR (qRT-PCR) and β-glucuronidase (GUS) reporter lines, we further demonstrated that CsGSTU8 was upregulated in response to drought stress and exogenous abscisic acid (ABA) treatments. Overexpression of CsGSTU8 in Arabidopsis resulted in enhanced drought tolerance as indicated by the improved scavenging of excess amounts of reactive oxygen species (ROS) under drought conditions. Furthermore, we found that CsWRKY48 acts as a transcriptional activator and that its expression is induced in response to drought stress and ABA treatment. Electrophoretic mobility shift assays (EMSAs), dual-luciferase (LUC) assays and transient expression assays in tea plant leaves revealed that CsWRKY48 directly binds to the W-box elements in the promoter of CsGSTU8 and activates its expression. Taken together, our results provide additional knowledge of drought stress responses in tea plant.
Grape anthracnose, which is caused by Elsinoë ampelina, is a disease that negatively affects grape production. This study aimed to investigate the effects of aeration, temperature, light, and preculture period on the formation of E. ampelina conidia and conidial germination and virulence. The colony morphology on potato dextrose agar (PDA) plates was more diverse than that in PDA bottles. The assessment of different culture methods, temperatures, light conditions, and preculture periods revealed that optimal conidial production occurred on 25‐day‐old colonies grown in PDA bottles at 21°C for 24 hr in the dark. The cultures in PDA bottles consistently produced approximately 5.0 × 106 conidia under these conditions. No conidial formation occurred when the cultures were kept at 25°C in the dark. The highest germination rate of E. ampelina was 80% at 25°C after 24 hr, whereas no germination was observed at 17°C after 12 hr. Pathogenicity tests revealed that symptoms of the disease were observed 4 days postinoculation (dpi) on leaves of Vitis vinifera cv. Red Globe. New conidia were observed on the lesions at 8 dpi. This study provides an effective method for the conidial production of E. ampelina that may also be applicable for other Elsinoë fungal species.
Drought stress severely limits growth
and causes losses in the
yield of tea plants. Exogenous application of 24-epibrassinolide (EBR)
positively regulates drought responses in various plants. However,
whether EBR could contribute to drought resistance in tea plants and
the underlying mechanisms has not been investigated. Here, we found
that EBR application is beneficial for the drought tolerance of tea
plants. The transcriptome results revealed that EBR could contribute
to tea plant drought resistance by promoting galactinol and abscisic
acid (ABA) biosynthesis gene expression. The content of galactinol
was elevated by EBR and EBR-responsive CsDof1.1 positively regulated
the expression of the galactinol synthase genes CsGolS2-1 and CsGolS2-2 to contribute to the accumulation
of galactinol by directly binding to their promoters. Moreover, exogenous
EBR was found to elevate the expression of genes related to ABA signal
transduction and stomatal closure regulation, which resulted in the
promotion of stomatal closure. In addition, EBR-responsive CsMYC2-2
is involved in ABA accumulation by binding to the promoters CsNCED1 and CsNCED2 to activate their expression.
In summary, findings in this study provide knowledge into the transcriptional
regulatory mechanism of EBR-induced drought resistance in tea plants.
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