The objective of this study was to determine the irritative potential of JP-8, S-8 and a 50/50 volume/volume mixture of JP-8 and S-8 following a single exposure to the skin of albino rabbits. Three groups of three albino rabbits received a single, four-hour, occluded exposure and an additional three groups received a single, four-hour, semi-occluded exposure. Doses (0.5 mL) of the test substances were applied to clipped, unabraded skin. At the completion of all exposures, the bandages were removed and the sites washed. Application sites were evaluated at approximately 30 to 60 minutes; 24, 48 and 72 hours; and 4, 7 and 14 days after patch removal for sites where irritation persisted. Occluded exposures of JP-8 or S-8 resulted in a classification of moderately irritating. Semi-occlusion of either JP-8 or S-8 produced slight irritation. The mixture, however, was slightly irritating in both the occluded and semi-occluded exposures.

No abstract

In a previous subchronic neurotoxicity study, increases in motor activity were observed for female rats after 9 and 13 weeks of exposure to 5000 ppm of isopropanol vapor.1 The present study was conducted to evaluate the reproducibility of these effects and, if reproducible, to assess the potential for reversibility following cessation of exposure. Two groups, each containing 30 female Fischer 344 rats, were exposed to concentrations of zero (control) and 5000 ppm of isopropanol vapor for 6 h per day, 5 days per week. Fifteen of the animals in the control and 5000 ppm groups were exposed for 9 weeks (designated as the 9‐week subgroup), while the other 15 animals in each group were exposed for 13 weeks (designated as the 13‐week subgroup). Motor activity was assessed for both subgroups prior to exposure and following 4, 7 and 9 weeks of exposure. Motor activity was also measured for rats in the 13‐week subgroup following 11 and 13 weeks of exposure. These motor activity measurements were made 18–20 h following the end of the last exposure for that week. In addition, to evaluate the reversibility of motor activity effects, measurements were made on three occasions during the week following the final exposure for rats in both the 9‐week and 13‐week subgroups and weekly thereafter for five additional weeks for rats in the 13‐week subgroup. Increases in cumulative test session motor activity counts were observed following 4, 7 and 9 weeks of exposure for rats in the 9‐week subgroup. Increases in cumulative test session motor activity counts were also observed following 4, 7, 9, 11 and 13 weeks of exposure for rats in the 13‐week subgroup. Reversibility of this effect was observed for rats in the 9‐week subgroup within 2 days following the last exposure. Reversibility was also noted for rats in the 13‐week subgroup but not until Study Week 15 (2 weeks following the last exposure). Minor changes were observed in the shape of the motor activity habituation curves for isopropanol‐exposed animals in the 9‐week and 13‐week subgroups at ca. 50% of the measurement intervals beginning at Study Week 4. While most of these statistical changes were observed in conjunction with increases in cumulative test session motor activity, some were observed following time points where recovery of the cumulative test session motor activity counts had occurred. No change in the shape of the motor activity habituation curve was observed at 42 days following the last exposure, indicating that complete recovery of motor activity effects had occurred. Thus, repeated exposure of female rats to 5000 ppm of isopropanol produced reversible increases in motor activity. © 1998 John Wiley & Sons, Ltd.

This report is published in the interest of scientific and technical information exchange, and its publication does not constitute the Government's approval or disapproval of its ideas or findings.