Water is not always accessible for agriculture due to its scarcity. In order to successfully develop irrigation strategies that optimize water productivity characterization of the plant, the water status is necessary. We assessed the suitability of thermal indicators by infrared thermometry (IRT) to determine the water status of grapefruit in a commercial orchard with long term irrigation using saline reclaimed water (RW) and regulated deficit irrigation (RDI) in Southeastern Spain. The results showed that Tc-Ta differences were positive in a wide range of vapor pressure deficits (VPD), and the major Tc-Ta were found at 10.00 GMT, before and after the highest daily values of VPD and solar radiation, respectively, were reached. In addition, we evaluated the relationships between Tc-Ta and VPD to establish the Non-Water Stressed Baselines (NWSBs), which are necessary to accurately calculate the crop water stress index (CWSI). Two important findings were found, which include i) the best significant correlations (p < 0.005) found at 10.00 GMT and their slopes were positive, and ii) NWSBs showed a marked hourly and seasonal variation. The hourly shift was mainly explained by the variation in solar radiation since both the NWSB-slope and the NWSB-intercept were significantly correlated with a zenith solar angle (θZ) (p < 0.005). The intercept was greater when θZ was close to 0 (at midday) and the slope displayed a marked hysteresis throughout the day, increasing in the morning and decreasing in the afternoon. The NWSBs determination, according to the season improved most of their correlation coefficients. In addition, the relationship significance of Tc-Ta versus VPD was higher in the period where the intercept and Tc-Ta were low. CWSI was the thermal indicator that showed the highest level of agreement with the stem water potential of the different treatments even though Tc and Tc-Ta were also significantly correlated. We highlight the suitability of thermal indicators measured by IRT to determine the water status of grapefruits under saline (RW) and water stress (RDI) conditions.

BACKGROUND: Non-conventional water sources and water-saving techniques can be valuable in semi-arid regions, although their long-term effects on citrus quality are little known. This study evaluated the effects of irrigation with two sources, transfer water (TW) and reclaimed water (RW), combined with two irrigation strategies, full irrigation (FI) and regulated deficit irrigation (RDI), on fruit quality of mandarins and grapefruits during eight growth seasons. RESULTS: Reclaimed water irrigation in mandarin, without water restriction, influenced maturity index (MI) less than TW-FI, because titratable acidity (TA) increased to a greater degree than soluble solid contents (SSC).Nevertheless, juice quality standards were satisfied. Regardless of the irrigation treatment (FI or RDI), a trend towards increasing fruit weight was also detected with RW. In grapefruit, its rootstock (Citrus macrophylla) enhanced salinity resilience with respect to the rootstock of mandarin ('Carrizo' citrange) and, hence, MI was not affected by RW. The RDI strategy, without saline stress (TW-RDI), increased, to a similar degree, both SSC and TA in mandarin fruit, not affecting the MI. In grapefruit, the water stress of RDI did improve the MI due to the TA did not change and SSC increased significantly, the TA did not change. The combination of both strategies, RW-RDI, decreased the MI only in some years because TA increased proportionally more than SSC in mandarin. CONCLUSIONS:The medium-and long-term feasibility of using RW and RDI to irrigate citrus was demonstrated. However, they must be performed cautiously and with appropriate management to avoid damaging fruit quality as a result of phytotoxic elements. Grapefruit response to irrigation with RW and RDIUnlike grapefruit, the weight of RW (FI) treatment fruit did not increase during the long-term period because their yield was not affected by RW.

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Citrus species are frequently subjected to water and saline stresses worldwide. We evaluated the effects of diurnal changes in the evaporative demands and soil water contents on the plant physiology of grapefruit and mandarin crops under saline reclaimed (RW) and transfer (TW) water conditions, combined with two irrigation strategies, fully irrigated (fI) and non-irrigated (nI). The physiological responses were different depending on the species. Grapefruit showed an isohydric pattern, which restricted the use of the leaf water potential (Ψl) as a plant water status indicator. Its water status was affected by salinity (RW) and water stress (nI), mainly as the combination of both stresses (RW-nI); however, mandarin turned out to be relatively more tolerant to salinity and more sensitive to water stress, mainly because of its low hydraulic conductance (K) levels, showing a critical drop in Ψl that led to severe losses of root–stem (Kroot–stem) and canopy (Kcanopy) hydraulic conductance in TW-nI. This behavior was not observed in RW-nI because a reduction in canopy volume as an adaptive characteristic was observed; thus, mandarin exhibited more anisohydric behavior compared to grapefruit, but isohydrodynamic since its hydrodynamic water potential gradient from roots to shoots (ΔΨplant) was relatively constant across variations in stomatal conductance (gs) and soil water potential. The gs was considered a good plant water status indicator for irrigation scheduling purposes in both species, and its responses to diurnal VPD rise and soil drought were strongly correlated with Kroot–stem. ABA did not show any effect on stomatal regulation, highlighting the fundamental role of plant hydraulics in driving stomatal closure.

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