The aims of the study were (1) to compare the cure risk of intramammary treatment of pure platelet rich plasma (P-PRP) or cefquinome sulfate (CS) in cows with subclinical mastitis (SCM) caused by Gram-positive bacteria, evaluated via somatic cell count (SCC) and the microbiological analysis of milk; (2) to compare the inflammatory/anti-inflammatory response of mammary gland to both treatments through the analyses of interleukins (IL), interferon gamma (IFN-γ), and tumour necrosis factor alpha (TNF-α) in milk. A non-inferiority randomized clinical trial was conducted. The null hypothesis was that cure risk in the experimental group (P-PRP) was inferior to the reference group (CS). A total of 103 cows were selected according to SCC and presence of Gram-positive bacteria, 49 cows were treated with CS and 54 cows were treated with P-PRP after determination of its cellular and molecular quality control. Cure was assessed by milk analyses at day 21 and 22 after treatment. Cows that remained with SCM were retreated at day 26, and cure assessed at day 47 and 48. Overall, bacteriological cure was observed in 16 cows (30%) of the P-PRP group, and 35 cows (71%) in CS group. Staphylococcus aureus cure risk was higher in CS group, but inconclusive for Streptococcus spp. The mean SCC increased in relation to time only in the P-PRP group. A direct relation between time and treatment for IL-1, IL-2, and IL-6 was observed, while no differences were observed for IL-4. Furthermore, IL-1 and IL-2 increased in cows treated twice in both groups. IL-8, IFN-γ, and TNF-α showed a significant interaction between time and treatment. IFN-γ concentration was lower in the P-PRP group compared to the CS on days 0 and 22. Leukocyte counts were lower in P-PRP when compared to whole blood. TGF-β1 and PF4 concentrations were higher in platelet lysates in comparison to P-PRGS and plasma. Moreover, PDGF-BB concentration was significantly higher in platelet lysates in comparison to plasma. Results obtained in this study demonstrate that SCM treated with PRP showed a lower rate of bacteriologic cure when compared to animals treated with CS.
Mycoplasma spp. is reported as a highly contagious mastitis-causing bacteria in dairy cattle, without successful or low response to most common antibiotic treatments due to the lack of cell wall. In Colombia it has been reported in the Central Andean region during 2014. The aim was to estimate the prevalence of Mycoplasma spp. in bulk tank milk using microbiological and molecular diagnosis. A random longitudinal study enrolling 220 commercial dairy farms located in four provinces of the mid-western region of Colombia from four pasteurizer companies was performed. Bulk tank milk samples were collected once monthly for three months period for determining somatic cell count (SCC) and microbiological and molecular diagnosis of Mycoplasma spp. cultures were done without pre-enrichment procedures directly in mycoplasma agar with cefoperazone to inhibit growth of opportunistic microorganisms, plates were incubated under 37° C and atmosphere of 10% CO2 and inspected during a 10d period. Molecular analysis was done by a multiplex PCR using specific primers targeting the 16S-23S rARN gene of Mycoplasma spp. and from non-pathogenic bacteria occasionally found in milk. LnSCC average of included dairy farms was 6.19 x103 cells/mL, Mycoplasma spp. was not isolated during microbiological cultures, and no DNA belonging to the species was detected by PCR in the 220 bulk tanks milk, with an estimated prevalence lower than 2.3%. This finding shows that there is not microbiological or molecular evidence that demonstrates the presence of the pathogen in the milk from the mid-western region of Colombia at herd level.
RESUMENEl objetivo de este estudio fue evaluar el transporte de los novillos por medio de indicadores conductuales y fisiológicos, y su relación con el pH alto en la carne a las 24 horas (pH24) del sacrificio. Se evaluaron 56 novillos cebú (2.5 ± 0.2 años, 429.9 ± 29.3 kg) en cuatro viajes al centro de beneficio. El transporte se hizo bajo condiciones similares (ruta, camión y conductor), donde se evaluaron los indicadores conductuales (interacciones afiliativas y agonistas, posturas del cuerpo, dirección del cuerpo en el camión y exploración), indicadores fisiológicos de estrés y el pH 24 de la carne. No se observaron diferencias significativas en las variables conductuales y el pH 24 según los compartimentos del camión. Las interacciones afiliativas fueron más frecuentes que las agonistas. Las concentraciones sanguíneas del hematocrito, cortisol, glucosa y creatinaquinasa mostraron valores altos. El 63.5% (n=36) de las carcasas presentaron pH 24 >5.8. Los resultados indican que el transporte comercial con duración de 8 h fue un factor de estrés fisiológico para los novillos cebú, con implicaciones negativas sobre el alto pH cárnico. La ausencia de la mezcla social favoreció la cohesión y la estabilidad grupal.Palabras clave: calidad de carne; bienestar animal; presacrificio; estrés
ABSTRACTThe aim of the study was to evaluate the transport of steers by behavioral and physiological indicators and its relationship with high pH of the beef at 24 h after slaughter.
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