Summary. An example of persistent mixed‐field polyagglutination of the Tn‐type is described, with particular reference to the value of plant seed agglutinins in the elucidation of polyagglutinable states. Extracts of the seeds of Dolichos biflorus, Bauhinia variegata and Wistaria sinensis strongly agglutinate Tn‐polyagglutinable cells. Inhibition studies indicate that N‐acetylgalactosamine may be an important determinant of Tn‐specificity. Positively charged polymers such as polybrene aggregate normal erythrocytes but not those appreciably deficient in sialic acid such as T‐ or Tn‐polyagglutinable cells. This observation forms the basis of a simple method of separating polyagglutinable from non‐agglutinating cells in a mixed‐field Tn‐polyagglutination. There are indications that Tn‐polyagglutinability, haemolytic anaemia, leucopenia and thrombocytopenia may constitute a distinct haematological syndrome.

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Summary The red cells of a normal male blood donor, K.S., were first grouped as B but he was found to lack anti‐A in his serum. Closer investigation revealed that his red cells had very weak A activity, demonstrable only by absorption and elution of anti‐A. He is a non‐secretor of ABH and a secretor of Lea. Blood group A‐, B and H‐gene specified glycosyltransferases were detected in his serum. In contrast to the finding of a B antigen of normal strength on his red cells, the B transferase in his serum was only about 30% of the normal level and, despite the very weak A activity of K.S.'s red cells, the A transferase level was about 50% of that found in the serum of group A individuals with normal strength of A antigen. Moreover, the A transferase on the basis of its pH optimum, Km values for donor and acceptor substrates, activation by divalent cations, isoelectric focusing profile and capacity to convert O to A‐active cells, was characterized as the product of an A1 gene. A family study showed that K.S.'s wife is group A2 and that they have two sons, one group A2 and the other group B. The group B son is assumed to have inherited a B gene from the propositus but the level of B transferase in the son's serum is three times as high as that in his father's serum. The wife of the propositus and his group A2 son have normal A2 transferases in keeping with their A2 red cell status. The A2 son therefore appears to have inherited an A2 gene from his mother but neither the A1 nor the B gene shown to be carried by his father. The distribution of transferase activities in K.S.'s red cells differs from that in his serum. A level of B transferase within the normal range was found in his red cell membranes but a very low level of A transferase was detected. The discrepancies between the serum transferases and ABO‐red cell group, together with the pattern of inheritance within the family, led to a suspicion of chimaerism. This was confirmed by the finding of fibroblasts with the female 46XX karyotype in cultures of the propositus' skin. These results suggest that K.S. is a dispermic chimaera with two different cell lines of the genotypes BO and A1O or A1A1. The group A2 son is assumed to have inherited an O gene from his father. It seems probable that K.S.'s bone marrow and reproductive organs are comprised predominantly of the XY cell line which carries the blood group BO genotype whereas his skin and other tissues which contribute the A1 transferase to his plasma, are partly made up of the XX cell line which carries the blood group A1O or A1A1 genotype.

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Summary. A hitherto undescribed from of transient red cell polyagglutinability is described. It was shown to be different from T‐, Tn‐ and Cad‐polyagglutinability. The value of reagents obtained from plant seeds in the elucidation of polyagglutinability is again demonstrated.