Genetic and molecular results are here presented revealing that the dissonance (diss) courtship song mutation is an allele of the no-on-transient-A (nonA) locus of Drosophila melanogaster. diss (now called nonAdiss) was originally isolated as a mutant with aberrant pulse song, although it was then noted to exhibit defects in responses to visual stimuli as well. The lack of transient spikes in the electroretinogram (ERG) and optomotor blindness associated with nonAdiss are shown to be similar to the visual abnormalities caused by the original nonA mutations. nonAdiss failed to complement either the ERG or optomotor defects associated with four other nonA mutations. However, all four of these nonA mutants--which were isolated on visual criteria alone--sang a normal courtship song. nonAdiss complemented at least three of the nonA mutations with regard to the singing phenotype, as assessed by a new method for temporal analysis of the male's pulse song. Both visual and song abnormalities caused by nonAdiss were rescued by P-element-mediated transformation with overlapping 11 and 16 kilobase (kb) fragments of genomic DNA (originally cloned from the nonA locus by Jones and Rubin, 1990). Analysis of behavioral phenotypes in transformed flies carrying mutagenized versions of the 11 kb genomic fragment (in a nonAdiss genomic background) localized the rescuing DNA to a region containing an open reading frame that encodes a polypeptide (NONA) with similarity to a family of RNA-binding proteins. Immunohistochemical determination of NONA's spatial and temporal expression revealed that it is localized to the nuclei of cells in many neural and non-neural tissues, at all stages of the life cycle after very early in development. Genetic connections between the control of two quite different behaviors--reproductive and visual--are discussed, along with precedences for generally expressed gene products playing roles in specific behaviors.

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SUMMARYBody composition of 324 steers from Angus dams by ten different sire breeds was compared at constant age. Progeny of the large European breeds and the Friesian attained heavier fasted live weights and carcass weights than the local Angus and Hereford breeds. Friesian cross-breds had the lowest dressing percentage. The Blonde d'Aquitaine and Limousin crosses had the lightest bone weights and this was reflected in high meat yields. The European breeds had the leanest carcasses, the traditional beef breeds the heaviest subcutaneous fat weights and the dairy breeds the heaviest internal fat weights. The results are discussed in relation to overseas findings.

Growth and carcass data were obtained over five years from 17-20 month heifers and steers by six terminal sirebreeds and out of 11 breed-types of dam comprising purebred Angus controls and 10 first-crosses (all half Angus). The slaughter cattle were sired by 38 separate bulls and the dams were sired by 99 sires from the Ruakura Beef Breed Evaluation scheme. Overall, 569 carcasses were analysed, with the left side being quartered and then dissected into meat, bone, and fat components. The terminal sire breeds were Charo1ais, Murray Grey, Red Devon, Simmental, and two others with small numbers. For live and carcass weights, calves sired by Charolais and Simmental bulls were 9-10% heavier than those by the Murray Greys, with those by Red Devons intermediate (5-70/0 above the Murray Greys, except at 12 months of age when they were 9.7% heavier). Calves sired by Charolais and Simmental bulls were leaner and their carcasses carried a greater proportional weight of meat than those by Murray Greys. However, calves sired by Murray Greys and Red Devons were similar in lean or fat levels. Three first-cross dam types of local origin were compared with purebred Angus, i.e., dams sired by Friesian, Hereford, or Jersey breeds. The remaining seven types were sired by the newer breeds, Blond d'Aquitaine, Charolais, Chianina, Limousin, Maine Anjou, Simmental, and South Devon. The dam types usually favoured for milk production (Friesian-, Jersey-, and Simmenta1

An experiment was carried out to study direct and indirect responses to selection in Romney sheep. There were two selection lines, one selected for greasy fleece weight (GFW) and the other for liveweight (LW), maintained alongside a control line (CO). Data from lambs born in 1967-90 were analysed to quantify the correlated responses to selection. By 1990, there had been an average of nine generations of selection with an average generation interval of 2.7 years. The correlated responses analysed included yearling and ewe greasy and clean fleece weights, washing yield, yearling and ewe fleece quality traits (staple length, mean fibre diameter, fibre diameter variation, loose wool bulk, Commission Internationale de l'Eclairange (CIE) Y value (brightness) and CIE Y-Z value (yellowness)), birth weight, weaning weight, yearling liveweight, ewe pre-mating weight, and ewe reproductive rate (fertility, litter size, weaning percentage, and lamb survival). Correlated responses were estimated as the deviation of selection lines from the CO line, and genetic correlations were obtained by restricted maximum likelihood techniques. Direct responses measured in the GFW and LW yearlings were 1.7 and 1.2% per year, respectively. Correlated annual responses were 0.7% in yearling liveweight in the GFW line and 0.2% in yearling fleece weight in the LW line. Corresponding annual changes in ewe performance were 1.5% in greasy fleece weight and 0.5% in pre-mating weight in the GFW line, and 0.0 and 1.2% respectively in the LW line. Mean fibre diameter increased in yearlings and ewes of the GFW line, but did not change in the LW line. There were small but significant increases in staple length in both lines. Loose wool bulk among GFW yearlings was reduced by 0.12% per year but did not change significantly in ewes. Yearlings and ewes of the LW line both showed an increase in bulk (0.15 and 0.43%, respectively). Relative to the CO flock, CIE Y-Z among yearlings increased significantly by 1.35% per annum and 0.25% per annum in the GFW and LW flocks respectively. Among ewes it increased in the GFW line whereas the opposite occurred in the LW line. There was no significant change in percentage ewes lambing as a result of GFW or LW selection. Litter size increased and lamb survival to weaning decreased significantly, but there was a net increase per year in lambs weaned per ewe lambing (0.27% per year for the GFW line and 0.48% per year for the LW line). A95024