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Objective: This study aims to prove that analyzing the hypervariable I (HV-I) and HV-II regions of mitochondrial DNA using 150 blood samples from Canis lupus familiaris would be an important source of evidence in solving an incident. Materials and Methods: The target region was amplified in the first and second polymerase chain reaction step following DNA isolation and the purification process was performed. As for the sequence analysis, it was analyzed in the ABI3130 capillary electrophoresis device. The analysis of raw data was performed. The results obtained were compared with the reference sequence. 16,658 (13.71%), and 16,676 (4.03%) of the region HV-II were identified for the first time. Conclusions: This study is a first of its kind in the field of forensic science in Turkey; however, the run time and cost were reduced via optimization in a different way as compared to the methods used in international publications.

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The study aimed to discover some variables influencing the risk of nicotine dependence in the Anatolian population. We examined 10 candidate genes and analysed 10 single nucleotide polymorphism (SNP) loci in 50 cases and 50 controls in a preliminary study. The main purpose of this work was to provide predicative definition in this regard. Buccal swab samples were collected from 50 cases and 50 controls, who had volunteered to participate in the study. The Fagerstr€ om Test for Nicotine Dependence (FTND) score of 4 or more was required for the case group. The control group was strictly chosen from people who had an FTND score of 0. SNP loci were genotyped and variations were observed in five SNP loci (rs16969968, rs806380, rs3733829, rs6474412, rs1329650). Based on these results, polymorphism was identified in the Turkish population in five SNP loci (rs16969968, rs806380, rs6474412, rs37333829, rs1329650). Although our findings indicated a non-significant association between the 10 selected loci and nicotine dependence in the Anatolian population, we believe that with a larger number of cases with five SNP loci, the variance we find will provide significant results for both forensic geneticists and psychiatrists. Since, to our knowledge, this is the first work to explore the potential association of these SNPs with nicotine addiction in the Anatolian population, the results should be expanded with larger sample groups.