Objective:The aim of this study is to evaluate the effects of platelet-rich plasma (PRP) on the proliferation, migration, and attachment of cultured periodontal ligament (PDL) cells.Materials and Methods:3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess number of PDL cells cultured in medium with or without PRP. Cell migration toward medium with or without PRP was assessed using the Boyden chamber. Cell attachment was assessed by counting cells on PRP or non-PRP coated dentin specimens. Group differences were analyzed using two-way ANOVA at 0.05 significance level.Results:In the MTT and cell migration assay, the number of cells in 5% and 10% PRP-treated groups were significantly higher than that in the non-PRP-treated group (P < 0.05). In the attachment assay, the number of cells on the dentin specimens in 10% PRP-treated group was significantly higher than that in the non-PRP treated group (P < 0.05).Conclusion:PRP could stimulate proliferation, migration, and attachment of PDL cells.

MU-IRB 2008/153.0511, ClinicalTrials.gov NCT00918060).

Objectives:Air polishing with sodium bicarbonate powders with a grain size of 40 μm is recommended for patient comfort. However, the efficacy of small grain size on stain removal has not been adequately studied. This study aimed to compare the stain removal efficacy of sodium bicarbonate powders with grain sizes of 65 and 40 μm and to evaluate patient acceptance and operator opinion after using both air polishing powders.Materials and Methods:A double-blind, randomized, split-mouth study was conducted with 35 participants with moderate to heavy dental staining on both sides of the upper teeth. Removal of dental stains on the index teeth was performed using sodium bicarbonate powders with a grain size of either 65 or 40 μm. The time taken to completely remove all dental stains was recorded. After treatment, a questionnaire was used to evaluate patient acceptance and the operator's opinion.Results:The average time for the removal of all stains by powder was 4.5 ± 3.6 min with a grain size of 65 μm and 4.4 ± 3.8 min with a grain size of 40 μm. The difference in the average time between the two groups was not significant (P = 0.461). The operator's opinions of the two powders were identical, and patient acceptance did not differ significantly between the two types of powders.Conclusions:The 40 μm sodium bicarbonate powder removed dental stains as efficiently as the 65-μm powder. Powder handling and patient acceptance were comparable between grain sizes of 65 and 40 μm.

This study aimed to evaluate the long-term outcome of the regenerative periodontal therapy of an intrabony defect in terms of tooth survival and clinical attachment level (CAL) stability. The association between failure and patient as well as tooth variables was assessed. Treatment records of the patients who received regenerative surgery and re-evaluation at 1-year post-surgery were screened. Patient and tooth variables, defect characteristics, and types of treatments were collected. Periodontal parameters were retrieved before regenerative surgery (baseline), 1-year post-surgery, and every visits of supportive periodontal treatment (SPT) until failure, including tooth loss or CAL loss ≥2 mm was found. In this study, treatment records from 89 patients were included. These patients continued SPT from 0.5-11.16 years. Of these patients, 92.1 % survived from tooth loss, while 61.8 % survived from CAL loss ≥2 mm compared to 1-year post-surgery. At the sites with residual pocket depth <5 mm, patients attending SPT >80 % had a significantly less percentage of teeth with CAL loss ≥2 mm compared to 1-year post-surgery than those attending SPT <80 %. However, at the sites with residual pocket depth ≥5 mm, no significant difference in the percentage of teeth with CAL loss ≥2 mm was found between patients attending SPT >80 % or <80 %. Smoking, patient's compliance, and residual pocket depth after regenerative surgery were significantly associated with tooth loss. However, these factors were not significantly associated with CAL loss compared to baseline or 1-year post-surgery.

No abstract