Abstract

Context: The possibility of hepatitis transmission through blood and blood products is very high and pretransfusion screening is mandatory by law in our country. The present study was carried out to find out the prevalence of Hepatitis B virus positive (HBsAg positive) among the blood donors attended the Department of Transfusion Medicine, Dhaka Medical College Hospital, Dhaka, in 2012. Method: This cross sectional study was carried out in the Department of Transfusion Medicine, Dhaka Medical College Hospital, Dhaka, between January and December 2012. Total blood donors were 27,560. Blood donors of 18-60 years of both sexes were included in this study. Results: Among 27,560 blood donors, relative blood donors were 21,081 (76.49%) and voluntary blood donors were 6,479 (23.50%). Among total blood donors male and female were 23,776 (86.26%) and 3784 (13.73%) respectively. 25,906 (93.99%) were in 18-40 years age group and 1,654 (6%) were in 41-60 years age group. A total of 453 were detected as HBsAg seropostive. Among them, relatives of the patients were 363 (1.7%) and voluntary donors were 90 (1.4%). DOI: http://dx.doi.org/10.3329/jdmc.v22i2.21528 J Dhaka Medical College, Vol. 22, No.2, October, 2013, Page 163-166

Dengue is the most common arthropod-borne sickness worldwide, impacting at least 50 million people each year. The dengue virus has four primary serotypes. Infection with one serotype confers homotypic immunity but not heterologous immunity, and secondary infections may be more severe. Although blood transfusions and organ donations have also been observed, the Aedes aegypti mosquito is the primary vector for the transmission of dengue. The infection causes a continuum of clinical illnesses, from asymptomatic infection to dengue fever, DHF, and dengue shock syndrome (DSS). To assess the presence of anti DENV IgG and anti DENV IgM antibodies specific to the four dengue serotypes in blood donor service donors and the importance of pre-donation screening in routine blood collection procedures.

Background: Dengue is the most common arthropod-borne sickness worldwide, impacting at least 50 million people each year. The dengue virus has four primary serotypes. Infection with one serotype confers homotypic immunity but not heterologous immunity, and secondary infections may be more severe. Although blood transfusions and organ donations have also been observed, the Aedes aegypti mosquito is the primary vector for the transmission of dengue. Infection causes a continuum of clinical illness, from asymptomatic infection to dengue fever, DHF, and dengue shock syndrome (DSS).Aim: To assess the presence of anti DENV IgG and anti DENV IgM antibodies specific to the four dengue serotypes in blood donor service donors and the importance of pre-donation screening in routine blood collection procedures.Method: 3 mL of peripheral venous blood from 507 blood donors was collected in tubes with BD vacutainer gel tube for serum separation after epidemiological records were reviewed. After that, serum was separated and tests were performed by SD Bioline Dengue Duo. Participants in the study completed a social and epidemiological questionnaire that contained information such as age, gender, and dengue diagnosis.Result: Out of the 507 blood samples that were taken, 473 (93.3%) came from male blood donors, while the remaining 34 (6.7%) belonged to female blood donors. The ratio of males to females is 13.91 to 1. The age range is 18–60 years, and the mean and standard deviation are both 27.7 and 6.5. 183 of the 507 samples produced anti DENV IgG positivity, while 324 did not. The ratio of positive to negative was 1.25:2.Conclusion: According to the findings of this study, quantitative methods for determining the presence of anti-dengue antibodies or detecting the dengue virus in blood donors in endemic areas should be devised in order to ensure the quality of blood transfusions.

Summary: Thalassaemiais a congenital hemolytic disorder caused by partial or complete deficiency of ? or ? globin chain synthesis. The disease is treated by lifelong red cell transfusion. So they are at high risk of developing allo-antibodies, resulting difficult to obtain compatible blood, results in, haemolysis & occasionally life threatening events.Objectives: To find out the frequency of Rhesus alloantibodies among the thalassaemic patients.Methods: This cross sectional study was done in the department of transfusion medicine in Bangabandhu Sheikh Mujib Medical University from 01-07-2010 to 03-07-2011. Sample size was 100 and the data was collected by face to face interview. Purposive sampling method was applied using inclusion & exclusion criteria. Statically analysis of the result was obtained by using window based computer software devised with Statistical Packages for Social Sciences (SPSS-15) (SPSS Inc, Chicago, IL, USA).Result: In the present study all respondent were Rh D-positive. Out of 100 respondents 2 (2.0%) had positive direct Coomb’sand 6 (6.0%) had positive indirect Coomd’s test. Out of 6 respondent with Rhesus all-antibody, 4 (66.7%) had anti E antibody, 1(16.7%) had anti c antibody and 1 (16.7%) had non specific antibody.Conclusion: In this study among the 100 respondents of thalassaemic patients who receive regular blood transfusion 6 (6.0%) had developed rhesus allo-antibody among with the most common was Anti E antibody 4 (66.7%). Such information may enable understanding and prevention of this serious and common complication.J MEDICINE July 2016; 17 (2) : 79-83