Background/AimsNeurofilament light chain (NfL) levels in cerebrospinal fluid and serum are reliable indicators for neuroaxonal damage in a broad spectrum of neurodegenerative diseases. Herein, we investigate NfL levels in serum and anterior chamber fluid of patients with glaucoma.MethodsPatients scheduled for routine glaucoma or cataract surgery were recruited for this study. Retinal nerve fibre layer thickness was measured by optical coherence tomography (OCT, Heidelberg Spectralis). NfL levels in serum and in anterior chamber fluid were analysed with Simoa SR-X Analyzer (Quanterix; NFLIGHT, Lexington, Massachusetts, USA). T-test was used for parametric data and Mann-Whitney-U test for nonparametric data. Spearman’s rank-order correlation was used to investigate correlations. P values<0.05 were considered as statistically significant.ResultsSixty patients with glaucoma and 58 controls were enrolled. Serum NfL concentration of patients with glaucoma was similar to serum NfL concentration in controls (median (IQR); 22.7 (18.9) pg/mL vs 22.5 (24.0) pg/mL; p=0.763). A positive correlation of serum NfL with age was observed in both patients with glaucoma (r=0.77; p<0.001) and in the control group (r=0.82, p<0.001). In the anterior chamber fluid, the NfL concentration was substantially increased in patients with glaucoma compared with controls (20.7 (101.3) pg/mL vs 3.1 (2.9) pg/mL; p<0.001). Furthermore, we found a positive correlation of anterior chamber fluid NfL with preoperative intraocular pressure (r=0.39, p=0.003) and with retinal nerve fibre layer thickness (r=0.58, p<0.001).ConclusionNfL levels in anterior chamber fluid are elevated in patients with glaucoma and correlate with intraocular pressure and retinal nerve fibre layer thickness. The presented data strongly support anterior chamber fluid NfL as a new marker for glaucoma.

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Comparative strain and hybrid investigation of dominant kthals and reciprocal translocations induced to mouse spermatogonia. -Hereditas 87:219-226. Lund, Sweden. ISSN 0018-0661. Received September 6, 1977Dominant lethals and translocation induction were assayed in mice after 276 R spermatogonial X-irradiation. Inbred A/Jax strain matings showed a much higher foetal mortality than did the CBA strain matings both in controls and in irradiated groups. No significant irradiation induction of dominant lethals was found. No strain-by-treatment interaction in embryonic death was found for males of either strain when mated to CBA females. Neither did the dominant lethal study reveal any differences between the two types of reciprocal hybrids between the strains, mated to CBA females, albeit the irradiated F, ( N J a x x CBA) group showed a significant increase of foetal mortality compared to its control.The frequencies of spermatocytes with translocations after spermatogonial irradiation of CBA, A/Jax, F, (CBAX A/Jax) and F, (A/Jaxx CBA) males were 0.040, 0.044, 0.047 and 0.047 respectively.No translocations were found in the control males. The estimates of the amount of dominant lethals expected from these frequencies seem to be somewhat higher than the actual results.The variances for the means of spennatocytes with translocations were significantly higher for both types of hybrids than for both strains.

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