This study investigated the proteoglycan (PG)-dependent mechanism ofImmunofluorescent co-staining with antibodies against chlamydial LPS and heparin did not identify bacterial and heparin co-localization on Jurkat cells. We also confirmed that when C. pneumoniae was statically infected to human CD4 + peripheral blood lymphocytes known not expressing detectable level of heparin, the bacteria attached to and formed inclusion bodies in the cells. Thus, the attachment mechanism of C.pneumoniae to Jurkat cells with low PG expression is unique when compared withHEp-2 cells and potentially independent of GAGs such as heparin.

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