Two-week supplementation with Q-EGCG was effective in augmenting GOBA andin countering inflammation after 3 d of heavy exertion in trained cyclists.

The purpose of this study was to test the influence of 2.4 g/d fish oil n-3 polyunsaturated fatty acids (n-3 PUFA) over 6 wk on exercise performance, inflammation, and immune measures in 23 trained cyclists before and after a 3-d period of intense exercise. Participants were randomized to n-3 PUFA (n = 11; 2,000 mg eicosapentaenoic acid [EPA], 400 mg docosahexaenoic acid [DHA]) or placebo (n = 12) groups. They ingested supplements under double-blind methods for 6 wk before and during a 3-d period in which they cycled for 3 hr/d at ~57% W(max) with 10-km time trials inserted during the final 15 min of each 3-hr bout. Blood and saliva samples were collected before and after the 6-wk supplementation period, immediately after the 3-hr exercise bout on the third day, and 14 hr postexercise and analyzed for various immune-function and inflammation parameters. Supplementation with n-3 PUFA resulted in a significant increase in plasma EPA and DHA but had no effect on 10-km time-trial performance; preexercise outcome measures; exercise-induced increases in plasma cytokines, myeloperoxidase, blood total leukocytes, serum C-reactive protein, and creatine kinase; or the decrease in the salivary IgA:protein ratio. In conclusion, 6 wk supplementation with a large daily dose of n-3 PUFAs increased plasma EPA and DHA but had no effect on exercise performance or in countering measures of inflammation and immunity before or after a 3-d period of 9 hr of heavy exertion.

Quercetin is a flavonol with broad spectrum bioactive effects that include anti‐inflammatory, anti‐pathogenic, antioxidant activity, and immunoregulatory influences. We tested the influence of 1000 mg quercetin (Q) with or without 120 mg epigallocatechin 3‐gallate (EGCG), 400 mg isoquercetin, and 400 mg EPA‐DHA (Q‐EGCG) on changes in measures of immunity and inflammation before and after a 3‐day period of heavy exertion. 39 trained cyclists were randomized to placebo, Q, or Q‐EGCG, and ingested supplements in a double blinded fashion for 2 weeks prior to, during, and 1 week after a 3‐d period in which subjects cycled for 3 h/d at 57% Wattsmax. Blood and saliva samples were collected before and after 2 weeks supplementation, immediately following the exercise bout on the 3rd day and 14‐h post‐exercise. Two weeks supplementation resulted in a significant increase in granulocyte oxidative burst activity (GOBA) in Q‐EGCG relative to P and increases in plasma quercetin for Q and Q‐EGCG. Immediately after the 3rd exercise bout, significant decreases for C‐reactive protein, plasma IL‐6 and IL‐10 were measured in Q‐EGCG compared to P. Granulocyte colony stimulating factor (GCSF) and CRP were reduced in Q‐EGCG 14‐h post‐exercise. Two weeks supplementation with Q‐EGCG was effective in augmenting GOBA, and countering inflammation following 3 days of heavy exertion in trained cyclists. Grant support from Quercegen Pharma.

We tested the influence of 2.4 g/day n‐3 polyunsaturated fatty acids (n‐3 PUFA) over 6 weeks on exercise performance, inflammation, and immune measures in 23 trained cyclists before and after a 3‐day period of intensified exercise. Subjects were randomized to n‐3 PUFA (N=11) (2,000 mg eicosapentaenoic acid, EPA, 400 mg docosahexaenoic acid, DHA) or placebo (N=12) groups, and ingested supplements under double blinded methods for 6 weeks prior to and during a 3‐d period in which subjects cycled for 3 h/day at ~57% Wattsmax with 10‐km time trials iduring the final 15 minutes of each 3‐h bout. Blood and saliva were collected before and after the 6‐week supplementation period, immediately following the 3‐h exercise bout on the third day, and 14‐h post‐exercise. Supplementation with n‐3 PUFA significantly increased plasma EPA and DHA, but had no effect on 10‐km time trial performance, pre‐exercise measures, and exercise‐induced increases in plasma cytokines, myeloperoxidase, blood total leukocytes, serum C‐reactive protein, and creatine kinase, or the decrease in the salivary IgA:protein ratio. In conclusion, 6 weeks supplementation with n‐3 PUFAs increased plasma EPA and DHA, but had no effect on exercise performance or in countering measures of inflammation and immunity before or after a 3‐day period of nine hours of heavy exertion.Supported by a grant from Cooper Complete, The Cooper Aerobics Center, Dallas TX