A new technique for rapidly discriminating shapes and/or sizes of micrometre size particles which are spatially distributed on a large scale has been developed. The technique is based on multiplexed matched spatial filtering, which is a kind of Fourier holographic filtering technique. Using the technique, large view visualization of the spatial distribution and the spatial behaviour of specific particles (e.g. aerosols, allergen particles, red blood cells, etc) can be realized. To make the multiplexed matched spatial filter (MMSF), a new material for hologram recording has been applied. The material is a photoconductor plastic which is processed by a solvent vapour and a corona discharge. The method of hologram recording is a dry process, which processes the material in several minutes at the initial settings of the device. Therefore, the MMSF can be made very easily in a short time. In the research, the discrimination of spatial behaviour of moving particles of different shape and/or size has been carried out by the MMSF made by the photoconductor plastic material.

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A digital speckle system has been combined with holographic interferometry to improve the accuracy of reconstruction of density distributions. For the combined system, several beam splitters have been used to separate the laser beams from a continuous-wave laser so that measurements can be performed simultaneously by both of the experiments. While the projections of the digital speckle system express the integration of the density gradients, those of the holographic interferometry can be obtained from the integration of the density itself. Thus, the steep region of the projections for the fringe shift of the interferometry has been measured by the use of the digital speckle system more accurately whereas the interferometry has been used to measure the projections for other regions simultaneously. CO2 flow from a rectangular nozzle has been investigated for the proposed combined system.

The field of microfluidics is developing rapidly with advances in MEMS (micro electro mechanical system) and μ TAS (micro total analysis system) technologies. In various devices, controlling the flow rate of liquid or gas accurately at micro or nanoliter volume levels is required. In this work, the gas-liquid two-phase flow patterns in a microchannel T-junction have been examined. The two-phase flow patterns were observed in the T-junction under two different gas and liquid injection conditions. The flow patterns of liquid and gas in the microchannel after the T-junction were also observed and classified into three types depending on the flow rate, and a two-phase flow pattern map was constructed. The mechanism of bubble breakup at a micro T-junction was also experimentally and analytically investigated.