A major current problem in public health is the issue of antimicrobial resistance of Escherichia coli in humans and poultry. In Indonesia, multidrug-resistant E. coli are of specific concern since such E. coli may cause public health problems in humans. The prevalence of multidrug-resistant chicken E. coli strains and the E. coli resistance genes, which are tet(A) and tet(B) genes, were investigated in the present study. A total of 57 swabs were collected from layer and broiler breeder farms in West Java, Indonesia, and used in the experiment. Eighteen isolates were identified as E. coli by the disk diffusion method. The isolates classified as drug-resistant and intermediate were then identified using PCR for the antimicrobial resistance genes. The results showed that 18 isolates of E. coli from layerbreeder and broiler-breeder farms in West Java were resistant to ampicillin (100%), nalidixic acid (94%), tetracycline (88%), oxytetracycline (83%), gentamicin (27%), and chloramphenicol (22%). PCR identification of E. coli antimicrobial-resistant genes in 18 isolates showed tet(A) and tet(B) genes. This study reports antimicrobial resistance genes among E. coli on layer and broiler breeder farms in West Java. This present study showed that E. coli isolated from layers-breeder and broiler-breeder farms in West Java of Indonesia carried tet(A) and tet(B) genes, the multidrug-resistance genes.

Bali cattle are the primary genetic resources of Indonesia. Besides beef cattle are used as breeds, they are also distributed to all regions in Indonesia. One of the requirements for good quality of beef cattle is to be free from parasitic diseases. Bima Regency is one of the areas of Bali cattle population centers in West Nusa Tenggara, but the Bali cattle maintenance system in Bima Regency is generally still extensive, certainly increasing the potential for diseases. The disease can cause economic losses, disrupt the health of livestock and the health of people who consume meat derived from parasitic-infected animals. This study used 50 stool samples of Bali cattle aged 5 to 12 months taken randomly. The sedimentation and flotation method was used to identify nematode worm eggs. The parameters observed were gastrointestinal parasitic morphology, and then the data were analyzed descriptively. It was found two types of parasites that infected bali cattle in Bolo Sub-district, Ascaris spp, and Trichuris sp. As caris spp infected as many as 72% of 50 bali cattle faecal samples, 72% were infected by Trichuris sp., and mixture of Ascaris spp and Trichuris spp infected 54 % of the sampled cattle. The improvement of cage management and cattle maintenance system are necessary to implement. Those are by taking into high-quality nutrition feeding, regular anthelminthic treatment, and avoiding the feeding of green forage to avoid worm infection and re-infection.

The purpose of this study was to test the resistance and to detect antibiotic resistence encoding gene in E. coli bacteria from chickens in Bandung and Purwakarta livestock. 18 E. coli isolates were tested for antibiotics resistance using the disk diffusion method. Isolates that were categorized as resistant and intermediate to antibiotics, then polymerase chain reaction was utilized to detect the resistent coding gene. The test results showed that all E. coli isolates from chickens in Bandung and Purwakarta were resistant to ampicillin (100%). E. coli isolates were still sensitive to chloramphenicol (11.1%) and gentamicin (22.2%). The gene encoding for ampC resistance from the test were in the amount of 77.7%. Sensitivity test results and detection of resistance coding gene showed that almost all isolates were resistant to ampicillin antibiotics and E. coli isolates were still sensitive to chlorampenicol and gentamicin.