Detection of Ampicillin Resistance Encoding Gene of Escherichia coli from Chickens in Bandung and Purwakarta

Khoirani, Kuntum; Indrawati, Agustin; Setiyaningsih, Surachmi

doi:10.20956/jrvi.v3i1.6134

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Keberadaan gen ampC ini terdeteksi dari isolat yang menunjukkan resistansi pada Ampisilin. Hal ini sejalan dengan laporan Indrawati et al, (2019) dan Khoirani et al, (2019) 1(8,3%) AML-AMP-E-S-TE Keterangan: AML: Amoksisilin; AMP: Ampisilin; E: Eritromisin; S: Streptomisin; TE: Tetrasiklin; SXT: Trimetoprim-sulfametoksazol juga berhasil mendeteksi gen ampC dari isolat yang resistan terhadap Ampisilin. Keberadaan gen blaTEM dan ampC yang merupakan gen pembawa ESBL mengindikasikan adanya ancaman penyebaran gen pembawa ESBL kepada bakteri lain melalui transfer gen resistansi secara horizontal.…”

Section: Gen Penyandi Resistansi Antibiotikunclassified

Deteksi Pola Resistansi dan Gen Penyandi Resistansi Antibiotik pada Escherichia coli Asal Peternakan Babi di Kabupaten Badung Provinsi Bali

Ardiana,

Indrawati,

Mayasari

2023

jveteriner

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Resistansi antimikrob merupakan masalah kesehatan global. Antimikrob termasuk antibiotik digunakan untuk mengobati infeksi pada manusia dan hewan. Penyalahgunaan antibiotik dapat menyebabkan resistansi dan pengobatan menjadi tidak efektif. Penelitian ini bertujuan untuk mengisolasi Escherichia coli (E. coli) dari hewan, manusia dan lingkungan di peternakan babi di Kabupaten Badung Provinsi Bali, serta menentukan pola resistansi dan gen resistansi antibiotik. Sampel diambil dari 12 peternakan babi yang berdekatan dengan total 24 sampel feses babi,24 sampel swab tangan pekerja kandang, dan 12 sampel limbah air. Escherichia coli diisolasi menggunakan MacConkey Agar, pewarnaan Gram, IMViC (Indole, Methyl Red, Voges Proskauer, Citrate) dan dikonfirmasi dengan Polymerase Chain Reaction (PCR). Uji kerentanan terhadap 11 antibiotik menggunakan metode difusi cakram. Deteksi gen resistansi antibiotik dengan PCR untuk gen blaTEM, ampC, ermB, strA, tetA, dan sul1. Hasil menunjukkan 100% (24/24) E. coli diisolasi dari feses, 16,7% (4/24) dari swab, dan 100% (12/12) dari limbah air. Pola resistansi antibiotik menunjukkan bahwa resistansi tertinggi terhadap eritromisin, diikuti oleh amoksisilin dan ampisilin, tetrasiklin, streptomisin dan trimetropim-sulfmetokzasol, serta 14 isolat dikategorikan Multidrug Resistant (MDR). Gen resistansi yang dideteksi dari E. coli pada manusia, hewan dan lingkungan adalah blaTEM, ampC, strA, tetA dan sul1, sementara ermB tidak terdeteksi. Berdasarkan hasil penelitian, pemberian antibiotik eritromisin, amoksisilin, ampisilin, tetrasiklin, streptomisin dan trimetropim-sulfmetokzasol sebaiknya diganti dengan antibiotik jenis lain. Adanya gen resistansi yang berasal dari sampel feses babi, swab tangan dan limbah air mengindikasikan terjadinya penyebaran resistansi antibiotik pada manusia, hewan dan lingkungan.

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Section: Gen Penyandi Resistansi Antibiotikunclassified

Deteksi Pola Resistansi dan Gen Penyandi Resistansi Antibiotik pada Escherichia coli Asal Peternakan Babi di Kabupaten Badung Provinsi Bali

Ardiana,

Indrawati,

Mayasari

2023

jveteriner

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Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria

et al. 2021

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Antimicrobial resistance (AMR) is a worldwide health problem affecting humans, animals, and the environment within the framework of the “One Health” concept. The aim of our study was to evaluate the prevalence of pathogenic strains of the species Escherichia coli (E. coli), their AMR profile, and biofilm-forming potential. The isolated strains from three swine faeces and free lagoons (ISO 16654:2001/Amd 1:2017) were confirmed using Phoenix M50 and 16S rDNA PCR. The antibiotic sensitivity to 34 clinically applied antibiotics was determined by Phoenix M50 and the disc diffusion method, according to the protocols of the CLSI and EUCAST. We confirmed the presence of 16 E. coli isolates, of which 87.5% were multi-drug-resistant and 31.25% performed strong biofilms. The possibility for the carrying and transmission of antibiotic-resistance genes to quinolones (qnr), aminoglycosides (aac(3)), β-lactamase-producing plasmid genes ampC, and blaSHV/blaTEM was investigated. We confirmed the carrying of blaSHV/blaTEM in one and ampC in seven isolates. The strains were negative for the virulence genes (ETEC (LT, STa, and F4), EPEC (eae), and STEC/VTEC (stx and stx2all)). The results should contribute to the development of effective measures for limitation and control on the use of antibiotics, which is a key point in the WHO action plan.

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Antimicrobial Resistance and Biofilm Formation of Escherichia coli Isolated from Pig Farms and Surroundings in Bulgaria

Kaleva,

Ilieva,

Zaharieva

et al. 2023

Microorganisms

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Escherichia coli (E. coli) is a ubiquitous microorganism with pathogenic and saprophytic clones. The objective of this study was to evaluate the presence, virulence, antibiotic resistance and biofilm formation of E. coli in three industrial farms in Bulgaria, as well as their adjacent sites related to the utilization of manure (feces, wastewater in a separator, lagoons, means of transport, and soils). The isolation of single bacterial cultures was performed via standard procedures with modifications, and E. coli isolates were identified via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR). The disk diffusion method was used to assess antimicrobial resistance, and PCR was used to detect genes for antibiotic resistance (GAR) (qnr, aac(3), ampC, blaSHV/blaTEM and erm) and virulence genes (stx, stx2all, LT, STa, F4 and eae). The protocol of Stepanović was utilized to measure the biofilm formation of the isolates. A total of 84 isolates from different samples (n = 53) were identified as E. coli. Almost all demonstrated antimicrobial resistance, and most of them demonstrated resistance to multiple antibiotics from different classes. No virulence genes coding the Shiga toxin or enterotoxins or those associated with enteropathogenicity were detected. No GAR from those tested for quinolones, aminoglycosides and macrolides were found. However, all isolates that were resistant to a penicillin-class antibiotic (56) had β-lactamase-producing plasmid genes. All of them had ampC, and 34 of them had blaTEM. A total of 14 isolates formed strongly adherent biofilms. These results in a country where the use of antibiotics for growth promotion and prophylaxis in farms is highly restricted corroborate that the global implemented policy on antibiotics in human medicine and in animal husbandry needs revision.

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Detection of Ampicillin Resistance Encoding Gene of Escherichia coli from Chickens in Bandung and Purwakarta

Cited by 4 publications

References 6 publications

Deteksi Pola Resistansi dan Gen Penyandi Resistansi Antibiotik pada Escherichia coli Asal Peternakan Babi di Kabupaten Badung Provinsi Bali

Deteksi Pola Resistansi dan Gen Penyandi Resistansi Antibiotik pada Escherichia coli Asal Peternakan Babi di Kabupaten Badung Provinsi Bali

Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria

Antimicrobial Resistance and Biofilm Formation of Escherichia coli Isolated from Pig Farms and Surroundings in Bulgaria

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