Scabies is a zoonotic skin disease caused by Sarcoptes scabiei mites. As an emerging/re-emerging parasitic disease, scabies represents a significant global threat to both human and animal health. Numerous cases of scabies in

Aim: This study aimed to sequence the Cytochrome c oxidase (COX-1) gene sequence from mitochondrial DNA of Sarcoptes scabiei isolated from Lamongan goats and Mojokerto rabbits, align it with DNA isolated from Zi'gong rabbit (GenBank accession No. EU256389.1), and produce a phylogenetic analysis of S. scabiei COX-1 gene. Materials and Methods: S. scabiei mites were obtained from goats and rabbits, and DNA was extracted using QIAamp DNA Mini Kit. The forward and reverse primer sequences were designed based on the DNA sequence of an S. scabiei COX-1 gene isolated from the Zi'gong rabbit (5'-TCTTAGGGGCTGGATTTAGTATG-3' and 5'-AGTTCCTCTACCAGTTCCAC-3', respectively). To confirm sequencing output, the sequence resulting from the reverse primer was inverted and aligned to the sequence from the forward primer using Clone Manager Professional Version 9 for Windows (Scientific & Educational Software; http://www.scied.com). This alignment was subsequently used to build a phylogenetic tree, using the Neighbor- Joining method, in the MEGA6 program (https://www.megasoftware.net/). Results: Polymerase chain reaction (PCR) products from S. scabiei isolates from Lamongan goats and Mojokerto rabbits produced bands of around 290 bp with 2% agarose gel electrophoresis. Comparing the DNA sequences of the S. scabiei COX-1 gene with those isolated from Lamongan goats and Mojokerto rabbits showed 99% homology. Conclusion: PCR products of the S. scabiei COX-1 gene isolated from Lamongan goats and Mojokerto rabbits were around 290 bp long. The sequences had more than 99% homology. The sequences of the COX-1 gene of S. scabiei from Lamongan goats and Mojokerto rabbits were relatively close to the sequence of the gene in S. scabiei obtained from various hosts according to National Center for Biotechnology Information data.

SGOT and SGPT are two enzymes found in the liver in large amounts. Therefore, elevated levels of these two enzymes in the blood indicate liver disease. This study aims to identify liver disease in cats in Surabaya through the levels of SGOT and SGPT in the blood as the gold standard of diagnosis. Samples came from stray cats and domesticated cats of random age, breed, and sex. The blood samples collected were 62 samples, consisting of 33 domestic cats and 29 samples from stray cats. This study showed that from 33 samples of domesticated cats, 19 samples had higher than normal levels of SGOT, and from 29 samples of stray cats, 27 samples had higher than normal levels of SGOT. For SGPT levels, from 33 samples of domesticated cats, six samples had higher than normal levels of SGPT, and from 29 samples of stray cats, six samples had higher levels of SGPT than average. Data analysis used an independent sample t-test with SPSS for Windows with a signifi cance level of 0.05. The data analysis results showed no signifi cant diff erence, which means that the high levels of cat SGOT and SGPT enzymes did not signifi cantly aff ect the origin of the cat. Therefore, it can be concluded that high levels of SGOT and SGPT as the gold standard for detecting liver diseases can occur in all cats, including stray cats and domesticated cats.

Leptospirosis is an eminent diseases among human and animal health. As a zoonosis disease, the occurrence of leptospirosis is not clearly understood in animal. Furthermore, the lesion caused by Leptospira sp. is not well demonstrated. This study aimed to analyze the correlation between the result of serological test using microscopic agglutination test (MAT) and the representation of histopathological lesion in kidney from the cattle. This study used 28 samples consist of cattle serum and kidney organs. The serum was tested using MAT and kidney was tested using histopathology. The data was reported semi quantitatively and tested using Spearman test. The result showed that there is no correlation between the result of serological test to the representation of histopathological lesion from the kidney of cattle. It is supported by the coefficient correlation (0,05) and probability value p=0,78 (p≥0,05). In conclusion, the result of Leptospira sp. serological test either seropositive or seronegative uncorrelated to the representation of histopathological lesion from the cattle kidney.

Background: The causes of cases of Acute Moist Dermatitis (AMD) or also known as hotspots are numerous. Early AMD is accompanied by symptoms of pruritus or different behaviors triggered by itching such as scratching or licking. Many causes of pruritus in cats such as hypersensitivity dermatitis, ectoparasites, fungal infections, bacterial infections, or skin reactions to systemic diseases. Gradually clinical symptoms will occur alopecia and erythema because it is very itchy, moist and smells on the surface of the skin. Case Description: A male Persian cat, 1.6 years old and weighing 3.4 kg, presented with a history of pruritus, alopecia, erythema and wet skin in several locations, especially around the neck, and had been present for 3 months. Appetite to eat and drink is not very good because the cat is busy scratching and biting its fur due to excessive itching. Examination Results and Treatments: After clinical examination and microscopic examination of samples, the main trigger in this case was furmite in a cat, Lynxacarus radovskyi with secondary bacterial infection causing Acute Moist Dermatitis (AMD). Not only that, ectoparasites in this case also cause other systemic diseases, namely suspicion of blood parasites that cause thrombocytopenia as evidenced by the results of a Complete Blood Count (CBC), and is characterized by clinical symptoms of hematuria and epistaxis. This cat underwent intensive treatment for several weeks with several combinations of drugs such as antiparasitics, antibiotics. antihistamines, NSID and vitamins. Conclusion: This