No abstract

We have performed a mutational analysis of the promoter for the chicken erythroid-specific GATA-1 transcription factor, and have investigated in detail the interaction of the factor with an upstream auto-regulatory element (ARE). We find that a single proximal GATA binding site of the ARE is required for promoter activity in primary erythroid cells; however, this minimal promoter is inappropriately active in fibroblasts. At least two molecules of GATA-1 can interact with the ARE, and sequences outside of the consensus site appear critical for the transcriptional activity of the bound protein. Finally, we provide evidence for complex protein/DNA interactions at the ARE, including the ability of GATA-1 to bend DNA.

Single-gene albino mutants and single-gene reversions to full coat color on C57BL/6J and DBA/2J background, respectively, were compared to nonmutant mice of these strains for rate of conditioned avoidance learning and extinction, alcohol preference, susceptibility to audiogenic seizures, locomotor activity, and wall-hugging behavior. Albino C57BL/6J mice learned the avoidance CR more slowly, drank less alcohol, and were less active than pigmented C57BL/6J mice. Nondilute DBA/2J mice were more active than dilute DBA/2J mice. Comparison of all C57BL/6J and DBA/2J mice revealed that mice of the former genotype were less resistant to extinction, drank more alcohol, were more active, and showed more

FIG. 3.-An enlarged photograph of portions of two cockroach antenna segments. X280. in his brown armor, carries with him a workable version of the olfactory sense. He appears therefore to be employable in our search for the secrets of that sense. I The evaluation and reading of the records, and the statistical computations, were made by qualified technicians. BRAIN FUNCTION AND MACROMOLECULES, I.

No abstract