Typical Aeromonas salmonicida with similar biochemical characteristics to A. salmonicida from Atlantic salmon, was isolated from wrasse, Ctenolabrus rupestris (L.) and Centrolabrus exoletus (L.), stocked as cleaner fish with these salmon. Although no external clinical signs were apparent, localized bacterial microcolonies were observed in musele, gills, intestine, kidney and myocardial tissue. Mortalities attributed to A-salmonicida comprised 55% {n = 32) of total mortalities. No carriers of A. salmonieida were found in wild wrasse following stress testing. Although salmon post-smolts died when challenged with 1 X Kh'^ml"^ of a virulent strain, there were no mortalities in challenged wrasse. An oral route of infection is suggested rather than water-borne transfer as wrasse browsed on salmon mortalities. Wrasse were treated for A. salmonicida infection by injection with antibiotic and were also vaccinated, and in the latter case, elevation of antibody levels was notedof Edinburgh 81B, 185-197.
Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24°C
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