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The place of Ukraine in the systems of the biogeographic and ecoregion order of the world and the biogeographic organization of Europe are considered. The ecoregions are defined as objects of cross-border and international cooperation for protection and conservation of biodiversity and planning of global, regional and national natural protection systems. The review of ecoregions of Ukraine was proposed. 12 ecoregions were defined as existing on the territory of Ukraine. The terrestrial ecoregions are represented by European mixed forests, the Eastern European forest steppe, Pontic steppe, Crimean Submediterranean forest complex, Carpathian montane forests, Pannonian mixed forests. The freshwater ecoregions are represented by Central & Western Europe, Dniester – Lower Danube, Dnieper – South Bug, Crimea Peninsula, Don. The marine ecoregion plays an important role of the nature protection system of Ukraine. This ecoregion is the Mediterranean Sea Ecoregion. The Black and Azov seas belong to its composition. WWF has identified a list of Global 200 that contains 238 ecoregions (142 terrestrial, 53 freshwaters, 43 marine) priority for the protection of their habitat diversity and biodiversity. More than half of these ecoregions are marked as endangered. The habitats of two ecoregions from the Global 200 list are in Ukraine. These are the habitats of the terrestrial ecoregion European-Mediterranean Montane Forests (the mountainous territories of the Crimean and Carpathian regions) and the freshwater ecoregion Danube River Delta. The ecoregions of priority nature conservation in Ukraine are characterized. The features of international cooperation on the biodiversity conservation in these regions are analyzed. The Crimean sub-Mediterranean forest complex is designated as problematic ecoregion for international cooperation. The active participation of Ukraine in the international cooperation for the biodiversity protection in the Euro-Mediterranean mixed forest is outlined. The result of this collaboration is the transnational object «Ancient and Primeval Beech Forests of the Carpathians and Other Regions in Europe». The unique Crimean beech forests with oriental beech (Fagus orientalis), forest beech (Fagus sylvatica), and taurida beech (Fagus taurica) did not enter this object. International cooperation is not possible in annexed territories. Ukraine’s priority has identified the need to develop National Geospatial Data Infrastructure (NGDI). The NGDI is a prerequisite for fruitful international and eco-regional cooperation

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