‘Charlottetown 80’ and ‘Herta’ barley were grown in nutrient solution (pH 4.3) with 0, 4, 6, 8, 10, and 12 ppm of added aluminum, in peat at pH values of 4.3, 5.5, and 6.2 each with 0, 3, 6, and 12 ppm of added Al, and in mineral soil at pH values of 4.7, 5.5, and 6.5. Actual concentrations of aluminum ion in solution were 0, 0.7, 1.5, 2.2, 2.6, and 3.0 ppm. Using the yield of Charlottetown 80 at 0 ppm as l00%, the yields of tops for the above Al concentrations were 100, 100, 96, 93, 49, and 42% for Charlottetown 80 and 41, 49, 32, 31, 20, and 23%, respectively, for Herta. Corresponding root yields were 100, 112, 113, 111, 76, and 66% (Charlottetown 80) and 42, 48, 39, 32, 24, and 31% (Herta). Saturation extracts from peat (pH 4.3) contained 2.0 ppm Al which reduced the top and root yields to 56 and 43% and 55 and 43% for Charlottetown 80 and Herta, respectively. Heavy fertilization reduced root yields in unlimed soil and increased root yields in limed soil. Percent K, Ca, and Mg in tops and roots decreased while percent Al increased with increasing Al concentration. Both uptake and translocation of P decreased with increasing Al concentration.