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The researches of cultivation of winter wheat with application of the biologically active substance potassium humate and nitrogen fertilizers of different concentrations N20 and N40 and their interaction N20+GK and N40+GK showed that potassium humate, without being a mineral fertilizer, increased the crop capacity, nitrogen content in grain, mass proportion of grain protein, nitrogen content in grain protein, Grain nutrition and quantity of gluten were the same as nitrogen fertilizers in concentrations of N20 and N40, and had comparable values for grain protein mass fraction, nitrogen removal with grain protein, grain nutrition and quantity of gluten; it also showed decrease for yield and nitrogen removal with grain of the crop up to 12% under simultaneous action of N20+GK and N40+GK. The application of potassium humate is ecologically and economically profitable from all the presented options, moreover the value of the crop yield is comparable with the applied nitrogen fertilizers in the concentrations of N20 and N40. In view of economic component of agricultural production in conditions of forest-steppe zone of Middle Volga region at the present time intensive system of agriculture with elements of biologization is possible.