No abstract

This study aimed at investigating the effect of fermented dairy beverage with clove essential oil (CEO) on the levels of reactive oxygen species (ROS) through endogenous enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). For this, bioinformatic analysis was performed using the Genecards and String platforms. Headspace was used to analyze and confirm the compounds at low concentrations. Antioxidant activity was analyzed using the DPPH free radical sequestration method. For analysis of antioxidant activity, twenty-four male Swiss mice were divided into three groups and submitted histopathological analysis, analysis of CAT, SOD,GPx transcripts, and CAT and SOD enzyme activity in visceral adipose tissue were evaluated.The leader genes found were PIK3CD, PIK3CB, AKT1, and PIK3CA, as they had the highest WNL values. The beverage containing CEO showed higher antioxidant activity, with free radical scavenging capacity above 80%. In the in vivo analyses, it was possible to verify a reduction in the average adipocyte area size (μm2) between the groups that received fermented dairy beverage. Although functional studies have shown that the antioxidant enzymes, CAT and SOD, showed similar concentrations in visceral adipose tissue of the three groups, the expression levels of GPx1, CAT, and SOD were higher in group 2. Surprisingly, the group 3, that received the fermented dairy beverage with CEO had the lowest SOD concentration (p <0.05). Therefore, the antioxidant mechanism of the CEO can be mediated by the activation of the cell survival pathway PI3K/Akt and modulation of SOD1 and CAT enzymes by means of ROS reduction.

O presente estudo teve como objetivo verificar a presença dos genes mecA, mecA LGA251, blaZ em isolados de S. aureus resistente a antibióticos beta-lactâmicos, provenientes de amostras de vacas diagnosticadas com mastite subclínica de rebanhos do norte de Minas Gerais. Isolados previamente identificados através da detecção do gene femA e MALDI-TOF MS e resistentes a antibióticos do grupo beta-lactâmicos foram utilizados. Os isolados foram submetidos à extração de DNA e para o rastreamento dos genes de resistência utilizou se a técnica de PCR. Embora fenotipicamente resistente a oxacilina e cefoxitina nenhuma das linhagens apresentou resultado positivo para os genes mecA e mecALGA251 nos ensaios efetuados, a ausência destes não descarta a possibilidade da presença de outros genes de resistências nas propriedades rurais em estudo. Porém, a presença do gene blaZ foi verificada em 4 (12,90%) dos isolados em estudo.Os resultados destacam a necessidade do monitoramento e adoção de medidas de controle que evitem à disseminação desses genes que representam grande risco a saúde pública.