a b s t r a c tBackground: Malassezia species are considered opportunistic yeasts of increasing clinical importance. These lipophilic yeasts are associated with various human diseases, especially pityriasis versicolor (PV), a chronic superficial scaling dermatomycosis. Aims: The aim of this study was to isolate, identify and analyze the distribution of the different species of Malassezia in patients with PV in Rosario city (Argentina). Methods: A total of 264 clinical samples were studied. Isolates were identified on the basis of microscopic observation of cells, and physiological properties, such as the presence of catalase, ability to use Tween compounds, splitting of esculin, and morphology, color and precipitate production on chromogenic agar CHROMagar-Malassezia medium (CHROMM). Results: The highest prevalence of PV in this study was observed in the 25-to 45-year-old group. No differences were found in the development of PV between sexes. The most affected areas of body were the trunk and face. Malassezia sympodialis (51%) was the most commonly isolated species, followed in frequency by M. globosa (40%), Malassezia furfur (7%), Malassezia obtusa (1%) and Malassezia slooffiae (1%). Conclusions: The success for a correct identification of these yeasts is important to improve our knowledge about their epidemiological role in PV and also to detect the appearance of strains which are resistant to the commonly used antifungal drugs.

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Objective: The aim of this article is to present the development of a method of analysis of the process of psychoanalytic psychotherapy with children (MAPPP-C) that is relevant for the study of the psychotherapy process from the perspective of the object relations theory. The process was studied by analysing changes in child patients' anxieties, defence mechanisms, object relations and unconscious phantasies, in each psychoanalytic psychotherapy session and throughout their entire psychotherapy. Method: MAPPP-C is both a qualitative and quantitative method of analysis of child psychoanalytic psychotherapy processes. This article will describe the stages in the de-velopment of the method, how the indicators were determined, the units of analysis in the study, the development of analytical codes, the training of raters in this method and inter-rater reliability and agreement tests. Results: This article describes the tasks that compose MAPPP-C, examples of its application and presents some hypotheses that arose from the analysis of the pilot study. Discussion: The preliminary results show that MAPPP-C is a method that allows one to assess the psychotherapist's interventions and the patient's responses in order to enable the analysis of the psychotherapeutic process. The results of the reliability test of the method will allow it to be applied to other types of psychoanalytic psychotherapy with both children and adults.

A single case study for a psychoanalytic psychotherapy process in a girl and her mother is presented from the perspective of the Object Relations Theory framework. Of interest are the empirical findings of therapeutic change and the effectiveness of the tool using a method created by a team of researchers from a university chair. A qualitative and quantitative analysis of textual observational records of sessions was conducted using codes constructed ad hoc from specific manuals for children and adults. The results of the application of the method are contrasted with results from psychological assessment techniques. Therapeutic change indicators are linked to progress in the processes of symbolization; therapeutic efficacy is recorded when the psychotherapist’s interventions favour symbolization and mentalization processes.

Esta técnica[acilila la obserl'ación lIJicroscópica 'cIe elementosjúngicos en materiales clínicos, donde su presencia es escasa o no fácilmente detectable por las técnicas lradicionales. Se colocó una gota de la solución de blanco de Calco.fluor con una gota de KOH 0120%, sobre elllJaterial estudiado, exalllinándolo microscópicalllente con luz U. ]. .. : Los elementosjiíngicos presentaron unajluorescencia I'erde manzana, lo que facilitó su detección en micosis sistémicas y subcutáneas. Presenta lino sensibilidad silllilar a la coloración de GOlllori-Grocott, pero tiene /a I'entaja de ser mucho más simple. Se concluye qlle esta técnica es mucho más sensible y espec(fica que la observación microscópica directa.