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Environmental pollution is one of the major issues faced by all the countries throughout the world. To prevent the environment scarcity and crisis faced in day-to-day life due the increasing chemical industries, usage of chemicals and the e uents processed out after the treatment also consists of some trace elements in them. Hence the extraction of enzymes on natural basis forms an alternative criteria for the production of dye in order to reduce pollution which in turn helps to nourish and protect the environment for future generations. Hibiscus sabdariffa (L) has a rich source of anthocyanins which is further enhanced by callus production and synthesized by increasing the sucrose concentration. Anthocyanin pigments were extracted using acidi ed ethanol and the dye obtained was screened for GL-MS analysis and its dyeing process in textile industry. The study showed signi cance properties along with coloring nature on the clothes used. Color of anthocyanin pigment depends on pH maintained and also shows the adaptability towards the nature with varied environmental conditions Highlights Extraction of anthocyanin by different solvent to improve the intense of coloration Phytochemical evaluation of plant sample to know the commercial values and medicinal values Plant tissue culture and callus assisted anthocyanin induction Amalgamation of plant oriented dye application (cloth dye, lip balm, dye sensitized solar cell).

In the present study, to optimize the media for the production of bioactive compounds from Monochaetia karstenii was carried out and compounds were identified by GC-MS. M. karstenii was identified from infected Camellia japonica leaves by classical and molecular taxonomy. It was cultured in different media and determined their mycelial biomass and antibacterial activity. Further Maltose Maltose tartrate broth (MTB) was altered for its media components such as carbon, nitrogen, minerals, amino acids and vitamins sources and physical parameters like temperature, pH and incubation periods for growth and production of secondary metabolites from M. karstenii. The antimicrobial and antioxidant compounds were performed from three different solvent extracts (Chloroform, Dichloromethane and Ethyl acetate) of M. karstenii from optimized medium. M. karstenii had optimum growth in MTB showing mycelial growth of 13.16 g/L. The ethyl acetate extract observed significant antibacterial activity against Escherichia coli (21 mm), Staphylococcus aureus (20 mm) and Vibrio chloreae (18 mm). In-vitro antioxidant activity revealed that, the IC 50 values for 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant radical scavenging assay of 100.58 μg/ml, 140 μg/ml and 141.91 μg/ml from ethyl acetate extract respectively. Thus the antimicrobial and antioxidant activity of the fungal extract has been due to the presence of biocompounds such as cyclohexenone derivatives, cinnamic acid, isooxazoline 3-phenyl-benzodiazepine, 2-propenoic acid 3-phenyl-(E)-dodecene and 3-undecen -1-yne (E) were characterized by gas chromatography-mass spectrometry (GC-MS) and reported first time in M. karstenii. We conclude that M. karstenii possess excellent antimicrobial and antioxidant potential and can be exploited for the discovery of new drug molecules.

Turbinaria ornata, tropical brown algae found in the South paci c and Indian Ocean ecosystems. In accordance with recent studies, Turbinaria ornata J. has potent anti-in ammatory effects. Therefore, this study is aimed to explore the biological activities of ethanolic extract of Turbinaria ornata J by analyzing the presence of phytochemical components, antioxidant property, antimicrobial activity and the wound healing activity. From the results, phytochemical analysis of ethanolic extract of T. ornata showed the presence of alkaloids, saponins, oils, total phenolic and total avanoid content were estimated to be 0.683 Abs and 0.433 Abs respectively. Anti-oxidant activity of the ethanolic extract of T. ornata extract showed remarkable DPPH radical scavenging activity of about 58.8% at 200μg/ml and total anti-oxidant activity of 0.257 absorbance at 100μg/ml concentration, as compared to that of their respective controls. The ethanolic extract of T. ornata exhibited the maximum zone of inhibition against the clinical pathogens like Pseudomonas aeuruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans and Methicillin-resistant Staphylococcus aureus with their potent anti-microbial activity. Wound healing effects of the ethanolic extract of T. ornata was analysed by using Zebra sh model. The results showed the rapidand signi cant regeneration of the wounded caudal n on day 14. Therefore, the preliminary results of this study strongly supports the ethanolic extract of T. ornata may be effective in wound healing and regeneration of the wounded tissues. HighlightsAntioxidant and Antimicrobial activities ethanolic extract of T. ornata J. supports the in vitro biological properties.The ethanolic extract of T. ornata J. reveals the promising regenerative property in zebra sh model.T. ornata J. possess strong antioxidant, antimicrobial and regenerative properties.

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