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The distribution of tritium labelled atropine in the mouse has been studied with whole body autoradiography. The proportions of unchanged atropine in different tissues were established with paper chromatography. It was found that H3‐atropine is rapidly taken up by the iris and ciliary body, the lung, salivary glands and certain endocrine glands such as the pituitary, thyroid, parathyroid, pancreatic islets and adrenal medulla. It is also rapidly accumulated in the secretory epithelium of the stomach and secreted into the lumen. Except for the choroid plexus and epiphysis very low concentrations of radioactivity are present in the brain. Very little passed the placenta barrier into the foetus. Although atropine is extensively metabolized in the mouse almost all the radioactivity present in submandibular gland and stomach was in the form of unchanged atropine.

Three groups of rats were maintained on diets containing different proportions of trans fatty acids (0, 18.3 or 36.6% of the total fatty acids) for eight weeks. No differences in body weight were observed among the three groups, but the fat cell size, determined in epididymal fat, differed significantly between the controls and the rats fed diets containing trans fatty acids. The supernatant obtained by centrifuging homogenates of liver from the rats at 9000 X g (S-9 fraction) was used as an activator in a bacterial test for mutagenicity of 2-aminofluorene and aflatoxin B1 using Salmonella typhimurium strains TA 98 and TA 100, respectively. The mutagenicities of 2-aminofluorene in strain TA 98 and of aflatoxin B1 in strain TA 100 were significantly lower with the liver S-9 fraction from rats fed a diet containing 36.6% trans fatty acids than with the liver S-9 fraction from rats fed a control diet with no trans fatty acids.