Toxicity of 29 plasticizers to HeLa cells in the MIT-24 system

Ekwall, Björn; Nordensten, Christina; Albanus, Lennart

doi:10.1016/0300-483x(82)90002-6

Cited by 40 publications

(19 citation statements)

References 14 publications

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“…Table 1 shows the ID50 values obtained with these compounds. We noted that ATBC was more toxic than DBS to human KB, monkey Vero and dog MDCK cells (Table 1), a finding in agreement with the results of Ekwall et al (1982) who used human HeLa cells.…”

Section: Methodssupporting

confidence: 90%

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Acetyl Tributyl Citrate and Dibutyl Sebacate Inhibit the Growth of Cultured Mammalian Cells

Mochida

Gomyoda

Fujita

1996

Bulletin of Environmental Contamination and Toxicology

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Section: Methodssupporting

confidence: 90%

“…As the values we obtained, that is 72h-ID50 Ekwall et al(1982) who used human HeLa cells, our present cell culture system seems to be more sensitive.…”

Section: Resultsmentioning

confidence: 50%

Acetyl Tributyl Citrate and Dibutyl Sebacate Inhibit the Growth of Cultured Mammalian Cells

Mochida

Gomyoda

Fujita

1996

Bulletin of Environmental Contamination and Toxicology

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“…These two categories of compounds are expected to tolerate relatively high supersaturations without undergoing significant homogeneous nucleation. Based on our literature reviews we concluded that these compounds should all be sufficiently nontoxic for use as CPC working fluids (Love and Muir 1976;Ekwall et al 1982;Zeiger et al 1985;Scheuch and Stahlhofen 1987;Brain et al 1996;Myojo and Sugimoto 1997;HSDB 2007;US-EPA 2007).…”

Section: Resultsmentioning

confidence: 99%

Effect of Working Fluid on Sub-2 nm Particle Detection with a Laminar Flow Ultrafine Condensation Particle Counter

Iida

Stolzenburg

McMurry

2009

Aerosol Science and Technology

183

186

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The effect of working fluid on size-dependent activation efficiencies with the laminar flow ultrafine condensation particle counter described by Stolzenburg and McMurry (1991) was studied theoretically and experimentally. Criteria considered include tendency to avoid homogeneous nucleation within the condenser and toxicity. The working fluids that were identified have vapor pressures below that of butanol, so particles grow to smaller sizes and are more difficult to detect optically. Therefore we use a second, conventional CPC as a "booster" to grow particles to a detectable size. Experiments were performed to obtain the size-and material-dependent activation efficiencies for ethylene glycol, diethylene glycol, propylene glycol, oleic acid, and DOS. Using diethylene glycol and oleic acid, values of the 50% activation efficiency diameter, D p50 , for negatively charged particles generated by evaporating sodium chloride, ammonium sulfate, and silver were <1.2 (<0.8) nm , 1.4-1.5 (1.0-1.2) nm, and 1.9-2.0 (1.5) nm as mobility (mass) diameter, respectively. The stability of the UCPC for long-term operation using ethylene glycol and propylene glycol as working fluids was tested by monitoring the instrument's response to silver particles having size near D p50 in an air stream at 40-45% relative humidity. The performance was steady (±3%) for several days indicating the instrument performs stably during unattended operation for realistic atmospheric sampling conditions.

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“…Ekwall et al (1987) (Table 1) reveals that the short-term bovine sperm motion assay is at least as sensitive as the cited cell culture assays using an exposure time of 24 h. It is obvious that detailed comparisons of the reported midpoint toxicities are very difficult because of the different cell types, endpoints, and incubation conditions. Several authors investigated the relation between physicochemical properties of chlorophenols and their toxicity to various aquatic species (K6nemann & Musch, 1981;Saarikosky & Viluksela, 1982;Benoit-Guyod et al, 1984;Devillers & Chambon, 1986).…”

Section: Discussionmentioning

confidence: 99%

Bovine spermatozoa as anin vitro model for studies on the cytotoxicity of chemicals: effects of chlorophenols

1989

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The suitability of ejaculated bovine spermatozoa as an in vitro model for the assessment of the cytotoxic potential of chemicals was evaluated using several endpoints: swimming activity, adenine nucleotide content, membrane integrity and oxygen consumption. A series of chlorophenols inhibited sperm motion (motility and velocity) in a concentration-dependent manner. This could be determined quantitatively and reproducibly by means of videomicrography and automatic computer image analysis. The sperm immobilizing potency increased with increasing chlorination and was positively correlated with lipophilicity. Concentrations which reduced the percentage of moving sperm to 50% of controls ranged from 43 microM for pentachlorophenol (PCP) to 1440 microM for 4-monochlorophenol (4-MCP). Determinations of adenine nucleotides and percentages of viable cells revealed qualitative differences between the action of PCP and the lower chlorinated phenols. While the latter decreased the total adenine nucleotide contents and the percentage of unstained cells in parallel to motion inhibition, no such changes occurred after exposure to immobilizing concentrations of PCP. Penta-, tetra- and trichlorinated phenols stimulated cellular respiration, indicating their uncoupling activity, at concentrations lower than those necessary for motion inhibition. The results indicate that bovine spermatozoa may become a useful in vitro model for the toxicological evaluation of chemicals providing quantitative as well as qualitative data.

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Toxicity of 29 plasticizers to HeLa cells in the MIT-24 system

Cited by 40 publications

References 14 publications

Acetyl Tributyl Citrate and Dibutyl Sebacate Inhibit the Growth of Cultured Mammalian Cells

Acetyl Tributyl Citrate and Dibutyl Sebacate Inhibit the Growth of Cultured Mammalian Cells

Effect of Working Fluid on Sub-2 nm Particle Detection with a Laminar Flow Ultrafine Condensation Particle Counter

Bovine spermatozoa as anin vitro model for studies on the cytotoxicity of chemicals: effects of chlorophenols

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