In this study, the method of fluorescence spectroscopy was used to improve the diagnostics and prediction of sepsis, pyo-inflammatory diseases and postpartum endometritis. At the first stage of the study, the researcher explored the fluorescence spectra of dilutions of serum with centrifuged and non-centrifuged bacterial culture (6-day crop sugar broth with Staphylococcus aureus), distilled water, 20% albumin and sugar broth. The focus was on the influence of treatment, including infusion therapy, on the fluorescence spectral characteristics of a patient's serum. At the second stage, the method of fluorescence spectroscopy was used for the diagnosis of sepsis in vivo. At the third stage, the analyst scrutinized the peculiarities of pregnancy, childbirth, and the postpartum period (totally, 40 parameters) in patients with postpartum pyo-inflammatory diseases and in the control group.

Background: Poor treatment of burns can lead to sepsis and even death. Especially promising is the use of the method of fluorescence spectroscopy for its diagnostics. The aim of the research is to create a pathogenetic concept as the basis of a diagnostic and treatment model of purulent-inflammatory diseases and sepsis. Material and methods: The study lasted from 2001 to 2019 and included 4 stages. The experimental base of luminescent research at all stages was the luminescence laboratory of the Department of Experimental Physics of the Ivan Franko National University of Lviv. The study was performed by using optical monochromators MDR-2 and MDR-12. The excitation of the blood serum was performed with light with a wavelength of 280 nm, which corresponds to the glow region of human serum albumin. Results: The main indicators, used for the analysis, were the fluorescence intensity (I F) and the position of the maximum fluorescence band (λ max). The object of the study was samples of the blood serum of patients of the main and control groups. In the case of the presence of endogenous intoxication in the body, albumin binding centers are blocked by the products of bacterial metabolism and therefore such "pathological" albumin is unable to perform its functions, including transport and detoxification. Conclusions: The pathogenetic concept as the basis of the diagnostic and treating model of purulent-inflammatory diseases and sepsis was devised. In particular, the important role of albumin solution infusion in the treatment of the above-mentioned diseases was established.

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Background: The deep understanding of pathogenesis is a key moment in the formation of the modern strategy of modern medicine. We conducted the thorough analysis of the microscopic processes occurring in the bodies of patients with purulent-septic complications. The modified pathogenetic concept of the diagnostic and treatment model of diseases with septic complications is presented. The obtained information about the mechanisms of origin and development of these diseases is fundamentally important for finding the modern effective methods of treating patients. The aim of the research is to modify treatment tactics for patients with sepsis and burn injuries based on How to cite this paper: Kovalenko, O., Ostapiuk, L. and Voloshinovskii, A. (2023) Current Problems of the Diagnostics and Treatment of Sepsis and Burn Injuries: The