Propyl-propanethiosulfinate (PTS) is a component of Allium essential oils. This organosulfur molecule can be used as a feed additive to decrease the appearance of bacterial resistances caused by the residues of antibiotics. In previous in vitro genotoxicity studies, contradictory results were reported for PTS. In this work, the in vivo genotoxicity of PTS in male and female rats was assessed for the first time, following OECD (Organisation for Economic Co-operation and Development) guidelines. After oral administration (doses: 5.5, 17.4, and 55.0 mg/kg PTS body weight), a combination of the micronucleus (MN) assay (OECD 474) in bone marrow and the standard and enzyme-modified comet assay (OECD 489) was performed. After necropsy, histopathological studies were also carried out. The results did not show the in vivo genotoxicity of PTS at any doses assayed, revealed by the absence of increased MN, and DNA strand breaks or oxidative DNA damage in the standard and enzyme-modified comet assays. The histopathological study revealed that only the highest dose tested (55.0 mg/kg) in the liver and all dose groups in the stomach presented minimal pathological lesions in the organs studied. Consequently, the present work confirms that PTS is not genotoxic at the doses assayed, and it is a promising natural alternative to synthetic preservatives and antibiotics in animal feed.

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Esta obra se distribuye con la licencia Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional (CC BY-NC-ND 4.0.) Resumen Durante el segundo cuatrimestre del curso 2018/2019, se ha realizado un Ciclo de Mejora en el Aula (CIMA), en la asignatura de Quimioinformática, Investigación e Historia de la Farmacia, en concreto en la parte correspondiente a los seminarios de Toxicología. El objetivo para este curso era desarrollar clases dinámicas a modo de debate con los alumnos, mediante la realización de diferentes preguntas, de modo que los alumnos se implicasen en el desarrollo de las clases. La experiencia, así como el desarrollo de las clases ha sido muy gratificante gracias a la respuesta obtenida por los alumnos. Es mi intención, continuar con este tipo de dinámicas, para seguir estimulando a los estudiantes de cara a su aprendizaje.