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apresentadas, assim como a frequência alta de profissionais que apresentam irritabilidade ou falta de paciência relacionado ao exercício profissional no setor de Terapia Intensiva. Palavras-chave: Síndrome de Burnout. sintomas de Burnout. Estresse profissional.

Rock field plant species present several adaptations to the climatic characteristics and seasonality of this habitat. In this study, we evaluate under field conditions the seasonal changes in non-structural carbohydrate accumulation in tuberous organs of Gomphrena marginata. Tuber fragments of plants of G. marginata were sampled monthly for a year (August 2010 to July 2011) in a rock-field area in southeastern Brazil. Quantitative analyses of total fructose, fructo-oligosaccharides, fructo-polysaccharides, reducing sugars and free proline were carried out. Qualitative analyses of carbohydrates were also performed by thin layer chromatography (TLC) and high performance anion exchange chromatography (HPAEC/PAD). Soil moisture was evaluated by gravimetry and plant water status was evaluated by measuring the relative water content (RWC) and water potential (Ψ w) of underground organs. Throughout the analyzed period, a well-marked seasonality of fructan accumulation was observed in tuberous roots of G. marginata correlating significantly with seasonal changes in soil water availability. In the rainy period (October to February), the content of fructo-polysaccharides decrease while during the dry and quiescent growth period (April to September) a high accumulation of fructo-polysaccharides was observed without changes in the RWC of tuberous roots, suggesting that cell osmoregulation contributed to water status maintenance in G. marginata. Our study presents a second species of Amaranthaceae that accumulates fructans as its main reserve carbohydrate, which can be an important strategy for survival in an environment characterized by a long dry period.

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