Abstract. Animals, the silent sentinels, stand watch over the world's environmental health. Everyday, animals demonstrate intricate connections between them, us and our surroundings. Amphibians are vertebrates and include approximately 4400 existing species. Amphibians are in most cases, small, diverse and sensitive to environmental variability. They can be good indicators of habitat diversity, biological variety and local stressors on the environment. They are bathed in both water and air. They live outdoors on land and water and their skin, larvae and unshelled eggs are constantly exposed and in contact with the substances in their surroundings. We searched Web of Science and references of relevant publications to evidence the application of amphibians, especially Xenopus laevis as model animal in ecotoxicology.

The aim of the present study was to characterize and preliminary assess the cytotoxicity and genotoxicity of different PMMA-TiO2 nanocomposites suitable to be used for two-parts stereolithographic complete denture manufacturing. In order to improve mechanical and antibacterial characteristics of the materials for 3D printing dentures, two matrix materials consisting in commercially available solutions of poly(methyl methacrylate) (PMMA), referred as dent-PMMA respectively base-PMMA, have been doped with different amount of TiO2 nanoparticles: 0.4, 0.6, 1.0, and 4.0% by weight. The obtained nanocomposites were structural characterized and assessed for antibacterial activity, cytotoxicity and genotoxicity. The newly obtained nanocomposites presented a good inhibitory action against the considered bacterial species-Staphylococcus aureus, good biocompatibility under 4.0% by weight. The results of the preliminary studies on cytotoxicity and genotoxicity assessment recommends the use of 0.4% TiO2 dent-PMMA and base-PMMA for CAD/CAM additive manufacturing of two-parts complete dentures/overdentures.

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