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Synthesis of surface modified/magnetic nanoparticles has become a vital research area of material science. In the present work, iron oxide (Fe3O4) nanoparticles prepared by solvo-thermal method were functionalized by polydopamine, which was as a facile nanoplatform for immobilizing glycerine dehydrogenase (GDH). The obtained nanocatalyst enabled efficient glycerol dehydrogenation reaction. The results show that GDH immobilized on polydopamine coated iron oxide (Fe3O4@PDA@GDH) revealed better adoptability towards higher levels of pH comparative. Moreover, this biocatalyst can be easily recovered from the reaction system without centrifugation or filtration.

Additives and antibiotic abuse during food production and processing are among the key factors affecting food safety. The efficient and rapid detection of hazardous substances in food is of crucial relevance to ensure food safety. In this study, a water-soluble quantum dot with glutathione as a ligand was synthesized as a fluorescent probe by hydrothermal method to achieve the detection and analysis of H2O2. The detection limits were 0.61 μM in water and 68 μM in milk. Meanwhile, it was used as a fluorescent donor probe and manganese dioxide nanosheets were used as a fluorescent acceptor probe in combination with an immunoassay platform to achieve the rapid detection and analysis of enrofloxacin (ENR) in a variety of foods with detection limits of 0.05–0.25 ng/mL in foods. The proposed systems provided new ideas for the construction of fluorescence sensors with high sensitivity.

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