Infertility can be detected when the couples have not completed pregnancy after a year or more of normal coitus. So, in order to treat infertility, there are many supported reproductive techniques are in practice. The success rate of these techniques depends upon the way by which preparation of the paternal semen sample. Over the past 30 years, the manual has been standard as providing global standards and has been used extensively by research and clinical laboratories throughout the world. The spermatozoa of all placental (eutherian) mammals, including humans, are in a protective, no labile formal at ejaculation and are incapable of fertilization even if they are placed in direct contact with an oocyte. Accordingly, they must undergo a subsequent period of final maturation. during which they acquire the capacity to interact with the oocyte cumulus complex and achieve fertilization. In this article we tried to explain the most important analysis and techniques necessary for semen preparation to produce healthy and normal offspring.

Background During luxate injury many dental tissue will be involved including pulp, periodontal ligament ,mucosa, hard dental tissue and alveolar bone with affecting the nerve and dental blood supplement. Growth factors may involved in healing process after replantation of tooth in its socket and the  Vascular endothelial factor (VEGF )  is a potent one that increase angiogenesis and accelerate repairing  and healing of dental pulp and the supporting tissues. The study was designed to illustrate the effect of application of growth factor on healing process of luxate molar tooth of rat. Materials & Methods Twenty male Wistar rats with luxate lower right first molar teeth were included in this study. The control were injected  with normal saline (0.5μl ) around the apex of mesial root .Experimental  group injected with  VEGF (0.5μl ). Histological assessment of  physiological responses of (dental pulp, cementum, periodontal ligament (PDL) and alveolar bone in both control and experimental groups with Receptor activator of nuclear factor kappa-Β ligand (RANKL) expression  evaluation were recorded during the healing period in the examined tissue.

No abstract