We showed that miR-107 expression was decreased in osteosarcoma (OS) tissues and cell lines. miR-107 mimic significantly decreased OS cell proliferation and inhibited invasion and migration of OS cells. Inhibition of miR-107 expression notably promoted proliferation, invasion and migration of OS cells. In addition, miR-107 mimic inhibited EMT biomarkers and significantly increased apoptosis. miR-107 mimic significantly decreased the protein expression of β-catenin, Cyclin D1, and c-Myc, whereas increased GSK-3β protein expression. miR-107 mimic markedly reduced the luciferase activity of 3'UTR of β-catenin. Overexpression of β-catenin inhibited miR-107 mimic-induced decrease of cell proliferation, invasion and migration ability, and increase of apoptosis.

Background: Intradural lumbar disc herniation (ILDH) is special type of lumbar disc herniation in which the lumbar nucleus pulposus prolapses and enters the dura mater. ILDHs comprise 0.04-0.33% of all herniated discs. In most cases, the diagnosis could not be confirmed preoperatively by identifying the typical features of ILDH in radiological evaluation. In the current report, we present a case of ILDH at lumbar 2/3 level and discuss the clinical presentations, typical imaging features, treatments, and outcomes.Case Description: We describe a rare case of ILDH. The patient was a 65-year-old man with pain in waist and back, and with bilateral radiating pain of lower extremities and fatigue for more than 2 weeks.Magnetic resonance imaging (MRI) revealed a large round, low-density mass at lumbar 2/3, which was easily mimicked as an intradural spinal tumor lesion. While enhanced MRI showed the typical rim enhancement sign and "Hawk beak" sign. Due to progressive decrease in muscle strength in both lower limbs, posterior microscopically assisted laminectomy, dural incision of the lumbar 2/3 was performed. Pathological examination revealed degenerated fibrous connective tissue and cartilage tissue. The patient's lower back pain and radiating pain and numbness of both legs improved remarkably postoperatively, and he became asymptomatic at 3 months and 1-year postoperatively.Conclusions: ILDH is a rare intervertebral disc herniation in clinical practice. The pathogenesis of ILDH may be related to aseptic inflammatory edema, closely fixing of ventral dura and the posterior longitudinal ligament, repeated mechanical action and chemical corrosion. Typical rim enhancement sign, "Hawk beak" sign and "Y" sign are important features of MRI in diagnosing ILDH, and intraspinal gas is also helpful in computed tomography (CT) diagnosis of ILDH. Prompt microscopically assisted laminectomy, dura mater incision to remove the intradural disc, pedicle screw fixation regardless of fusion, surgical results are usually favorable. We also reviewed the literature and discussed the epidemiology, potential pathogenesis, diagnosis, treatment and poor prognostic factors of ILDH.

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Background Osteoporosis is prevalent among older adults but is an underdiagnosed condition. The purpose of this study was to explore the relationship between bone mineral density (BMD) and computed tomography (CT) attenuation based on dual-energy X-ray absorptiometry (DXA) as the reference standard and to predict the CT attenuation threshold for osteoporosis. Methods The cohort included 430 subjects (276 women and 154 men; mean age: 67.6 years) whose general information, CT attenuation, DXA, aortic calcification, and vertebral degeneration scores were collected. The relationship between DXA scores and aortic calcification and vertebral degeneration scores was analyzed by orderly logistic regression. The prediction of CT attenuation for osteoporosis was analyzed by receiver operating characteristic (ROC) curves. Results There was no significant difference in CT attenuation on the vertebral coronal, sagittal, or axial plane (p > 0.05). Obesity, paravertebral osteophytes, endplate sclerosis, and aortic calcification could impact the outcomes of DXA (p < 0.05). The sensitivity for distinguishing osteoporosis was at least 90% when the CT attenuation threshold was 120 HU; when the CT attenuation threshold was 81 HU, the specificity for distinguishing osteoporosis was at least 90%. When the threshold was 98 HU, the Youden index was the largest, with a specificity of 80.0% and a sensitivity of 82.3%. Conclusions CT attenuation of the lumbar spine can be used to identify patients with osteoporosis while reducing the influence of the bony structure around the vertebral body on the assessment of BMD.