The banding pattern of seed storage proteins resulting from sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) of extracts from bulked seed samples has been used to distinguish species and cultivars within the genera Latium and Festuca. Perennial (L. perenne L.) and Italian (L. multiflorum Lam.) ryegrasses were readily differentiated, and distinctive banding patterns were obtained for seed proteins from a range of cultivars and varieties of each, some very closely related. These patterns were independent of site, year, or generation of seed production. Fescue species differentiated were F. pratensis Huds. (meadow fescue), F. arundinacea Schreb. (tall fescue), and F. rubra L. (creeping red and Chewings fescues). The 2 types within F. rubra could be distinguished. SDS-PAGE of bulked seed samples offers a rapid method for distinguishing cultivars of Latium and Festuca during seed certification, for checking for genetic shift or contamination in cultiv.ars ~u~ng seed multiplication, as well as for Identlfymg unknown or wrongly named lines. Seed protein banding pattern has potential as a tool for taxonomic classification and could also be used as an adjunct to the more traditional chara~ters ~sed in distinguishing cui ti vars for Plant Vanety Rights.
Subterranean clover seed was collected from 51 old pastures in 9 regions of New Zealand. Spaced plants from these 51 populations were grown at Palmerston North and compared with a range of Australian commercial lines. Mt Barker types were present in every New Zealand population sampled and constituted 74% of the total collection. Tallarook and Woogenellup made up 21% and 2070 of the collection respectively. A very late-flowering dwarf type with Mt
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