Identification of Cultivars of Grasses and Forage Legumes by SDS-PAGE of Seed Proteins

Gardiner, Susan E.; Forde, M. B.

doi:10.1007/978-3-662-01639-8_3

Cited by 13 publications

(14 citation statements)

References 8 publications

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“…The SDS-PAGE is a promising tool for distinguishing cultivars of a particular crop species (Cooke, 1984;Gardiner and Forde, 1988). The present study was therefore undertaken in order to distinguish among the five given cultivars of mungbean through the use of SDS-PAGE.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Islam¹,

Mir²,

Kudesia³

2015

International J. of Plant Breeding and Genetics

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Seed storage protein profiling through SDS-PAGE and DNA Restriction Fragment Length Polymorphism (RFLPs) were surveyed in 5 accessions of mungbean (Vigna radiata L.) sampled from NBPGR Thrissur, Kerala, India, to evaluate genetic diversity. The protein profile showed a low level of polymorphism (26.66%). The similarity index calculated ranged from (42.30-50%). The dendrogram constructed revealed two clusters. Cluster 1st comprises two accessions IC 251432 and IC 251433. Cluster 2nd comprises 3 cultivars viz., IC 251431, IC 251430 and IC 251434. Cluster 2nd also revealed a 100% homology between IC 251431 and IC 251430 accessions. The RFLP analysis was carried out by single restriction enzyme EcoRI. The RFLP profile revealed a high level of polymorphism (90.90%). A total of 11 clear bands were scored. The only band with fragment size 630 kb was monomorphic while, the rest were polymorphic. Similarity index ranged from (42.82-11.11%). The UPGMA analysis divided the accessions into 2 clusters viz., cluster 1st comprising 4 accessions (IC 251431, IC 251432, IC 251433 and IC 251430). Cluster 2nd includes only one accession (IC 251434) occupying a distinct position in constructed dendrogram.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Islam¹,

Mir²,

Kudesia³

2015

International J. of Plant Breeding and Genetics

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“…A bulk sample is a composite seed sample that represents the mixture of electrophoretic phenotypes present in a cultivar. For bulk extraction, a set of at least 200 seeds (Gardiner & Forde, 1992) was used to obtain a representative sample, although our results showed that a batch of 50 seeds provided a reproducible banding pattern (results not shown). Electrophoregrams of the bulk samples were evaluated by the presence/absence of bands and by their intensities, distinguishing four levels from 1 to 4, corresponding to increasing band intensity.…”

Section: Single Seed and Bulk Analysismentioning

confidence: 90%

Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

Rogl

Javornik

1996

Euphytica

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Total seed proteins of 24 common buckwheat cultivars and cultivated populations within a molecular weight range of 30 to 54 kDa were analysed by SDS-PAGE. Single seed analysis of six cultivars identified a total of 18 alternative protein bands with different mobilities. Differences of individual protein band frequencies extracted from single seeds among six buckwheat cultivars varied distinctively, indicating high intravarietal polymorphism. The relation between frequencies of protein bands revealed by single seed analysis and their appearance on the bulk seed analyses was demonstrated. Regarding to band mobility rate and relative band intensity among 24 bulk samples analysed, 14 had distinctive electrophoregrams while the other 10 were ranged into four distinct groups. Analysis of endosperm and cotyledon proteins showed that proteins stored in these main seed parts are tissue specific. The observed electrophoretic polymorphism related to proteins stored in the cotyledons while there was no apparent variability with endosperm proteins.

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“…As a result, new procedures are needed to identify cultivars (14). Doyle et al (11) has successfully used DNA data in grass taxonomy and phylogeny.…”

Section: Introductionmentioning

confidence: 99%

“…Grass seed storage proteins have already been investigated systematically by several authors (16,26). Seed protein electrophoresis was found to be a useful technique for cultivar discrimination in several economically important grass species including Kentucky bluegrass (Poa pratensis l.) and ryegrass (L. perenne l. and L. multiflorum l.) (8,12,13,14,15,20,21,28). it is possible to identify and certify individual genotypes to analyze the progenies and populations (2,18,24,27).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Agronomic Characters, Total Seed Storage Proteins and their use for Genotypes Discrimination in the Kentucky Bluegrass (Poa PratensisL.)

Tamkoç

Arslan

2010

Biotechnology & Biotechnological Equipment

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Identification of Cultivars of Grasses and Forage Legumes by SDS-PAGE of Seed Proteins

Cited by 13 publications

References 8 publications

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Evaluation of Genetic Diversity in Vigna radiata (L.) Using Protein Profiling and Molecular Marker (RFLP)

Seed protein variation for identification of common buckwheat (Fagopyrum esculentum Moench) cultivars

Comparison of Agronomic Characters, Total Seed Storage Proteins and their use for Genotypes Discrimination in the Kentucky Bluegrass (Poa PratensisL.)

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